Fluorescence Intensity Enhancement of Green Carbon Dots: Synthesis, Characterization and Cell Imaging,Photochemistry and Photobiology

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Fluorescence Intensity Enhancement of Green Carbon Dots: Synthesis, Characterization and Cell Imaging
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2020-05-12 , DOI: 10.1111/php.13261
Fatemeh Hashemi _{1,

2} , Fatemeh Heidari _{3,

4} , Nasrin Mohajeri ₃ , Farideh Mahmoodzadeh ₅ , Nosratollah Zarghami _{1,

2,

3,

4}

Affiliation

The hydrothermal treatment of green carbon dots (CDs) is an appropriate fluorescent probe synthesis method. CDs are exploited as biological staining agents, especially for cellular detection and imaging. The nitrogen‐doped green carbon dots (N‐CDs) formation can improve the fluorescence intensity property in a one‐step process. Here, we report two N‐CDs from lemon and tomato extraction in the presence of hydroxylamine. Lemon and tomato N‐CDs showed the blue fluorescence under ultraviolet radiation of about 360 nm. The characterization of CDs and N‐CDs showed the presence of N‐H and C–N bonds which enhanced the fluorescence efficiency. The mean size of lemon and tomato N‐CDs were about 2 and 3 nm with an increased quantum yield (QY) of 5% and 3.38%, respectively. The CDs and N‐CDs cytotoxicity assay exhibited high cell viability approximately 85% and 73%, respectively. N‐CDs show superior fluorescent intensity in different solvents and significant stability under long‐time UV irradiation, different PH and high ionic strength. Our results indicated that the use of N‐CDs in cell imaging can lead to fluorescence intensity enhancement as well as proper biocompatibility. Therefore, the safe and high fluorescence intensity of green N‐CDs can be utilized for fluorescent probes in biolabeling and bioimaging applications.

中文翻译：

绿色碳点的荧光强度增强：合成、表征和细胞成像

绿碳点 (CD) 的水热处理是一种合适的荧光探针合成方法。CD 被用作生物染色剂，特别是用于细胞检测和成像。氮掺杂的绿色碳点（N-CDs）的形成可以一步法提高荧光强度特性。在这里，我们报告了在羟胺存在下从柠檬和番茄提取物中提取的两种 N-CD。柠檬和番茄 N-CD 在约 360 nm 的紫外线辐射下显示蓝色荧光。CDs和N-CDs的表征表明N-H和C-N键的存在提高了荧光效率。柠檬和番茄 N-CD 的平均大小约为 2 和 3 nm，量子产率 (QY) 分别增加了 5% 和 3.38%。CDs 和 N-CDs 细胞毒性试验分别显示出约 85% 和 73% 的高细胞活力。N-CDs在不同溶剂中显示出优异的荧光强度，并且在长时间紫外线照射、不同PH值和高离子强度下具有显着的稳定性。我们的结果表明，在细胞成像中使用 N-CD 可以增强荧光强度以及适当的生物相容性。因此，绿色 N-CD 的安全和高荧光强度可用于生物标记和生物成像应用中的荧光探针。我们的结果表明，在细胞成像中使用 N-CD 可以增强荧光强度以及适当的生物相容性。因此，绿色 N-CD 的安全和高荧光强度可用于生物标记和生物成像应用中的荧光探针。我们的结果表明，在细胞成像中使用 N-CD 可以增强荧光强度以及适当的生物相容性。因此，绿色 N-CD 的安全和高荧光强度可用于生物标记和生物成像应用中的荧光探针。

更新日期：2020-05-12

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