Crangon crangon is economically a very important species. Recently, promising culture attempts have been made, but a major problem is the uncontrollable mortality during the grow-out phase. As of yet, the life cycle of C. crangon is not closed in captivity so wild-caught individuals are used for further rearing. Therefore, it is important to investigate the virome of C. crangon both in wild-caught animals as in cultured animals. In recent years, next-generation-sequencing (NGS) technologies have been very important in the unravelling of the virome of a wide range of environments and matrices, such as soil, sea, potable water, but also of a wide range of animal species. This will be the first report of a virome study in C. crangon using NGS in combination with the NetoVIR protocol. The near complete genomes of 16 novel viruses were described, most of which were rather distantly related to unclassified viruses or viruses belonging to the Picornavirales, Bunyavirales Nudiviridae, Parvoviridae, Flaviviridae, Hepeviridae, Tombusviridae, Narnaviridae, Nodaviridae, Sobemovirus. A difference in virome composition was observed between muscle and hepatopancreatic tissue, suggesting a distinct tissue tropism of several of these viruses. Some differences in the viral composition were noted between the cultured and wild shrimp, which could indicate that in sub-optimal aquaculture conditions some viruses become more abundant. This research showed that a plethora of unknown viruses is present in C. crangon and that more research is needed to determine which virus is potentially dangerous for the culture of C. crangon.

中文翻译：

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探索欧洲棕色虾（Crangon crangon）的病毒。

克兰贡克兰贡在经济上是非常重要的物种。最近，已经进行了有希望的培养尝试，但是主要的问题是在成长期中无法控制的死亡率。迄今为止，圈养梭菌的生命周期尚未关闭，因此将野生捕获的个体用于进一步饲养。因此，重要的是在野生动物中和培养动物中研究克兰氏梭菌的病毒。近年来，下一代测序（NGS）技术在揭露各种环境和基质（例如土壤，海洋，饮用水以及各种动物）的病毒中非常重要。这将是C. crangon的一项病毒学研究的第一份报告。将NGS与NetoVIR协议结合使用。描述了16种新病毒的近乎完整的基因组，其中大部分与未分类病毒或属于Picornavirales，Bunyavirales Nudiviridae，Parvoviridae，Flaviviridae，Hepeviridae，Tombusviridae，Narnaviridae，Nodaviridae，Sobemovirus的病毒远缘。在肌肉和肝胰腺组织之间观察到病毒组成的差异，表明这些病毒中有几种具有明显的组织嗜性。在养殖的虾和野生的虾之间发现了一些病毒组成的差异，这可能表明在次佳的水产养殖条件下，某些病毒变得更加丰富。这项研究表明，在C. crangon中存在大量未知病毒，还需要更多的研究来确定哪种病毒对C. crangon的培养具有潜在危险。