Pigment from the endophyte Burkholderia sp. WYAT7 isolated from the medicinal plant Artemisia nilagirica (Clarke) Pamp. was extracted. The antibacterial efficacy of the crude pigment Y was assessed as a source of antibiotic against both Gram-positive and Gram-negative bacterial pathogens. The pigment Y exhibited a significant level of antibacterial activity against the tested pathogens Salmonella typhi (MTCC 733), Staphylococcus aureus (MTCC 1430), Pseudomonas aeruginosa (MTCC 2453), Klebsiella pneumoniae (MTCC 432), Escherichia coli (MTCC 1610), Salmonella paratyphi (3220), Bacillus subtilis (441) and Acinetobacter baumannii (12,889). The minimum inhibitory concentration of crude pigment extract Y for most of the bacterial pathogens tested was below or equal to 0.25 µg/mL and the minimum bactericidal concentration was below or equal to 0.5 µg/mL. In the cytotoxicity evaluation, crude pigment Y exhibited less toxicity toward normal cells lines (L929). Crude pigment extract Y also showed powerful anticancer activity toward melanoma cancer cells (A375). The IC50 value obtained was 68.08 µg/mL. Acridine orange (AO) and ethidium bromide (EB) double staining of cells treated with the pigment helped in the morphological assessment of nuclear condensation, apoptotic bodies and live cells. The DNA fragmentation analysis and caspase-9 quantification in the pigment-treated A375 cells determined the apoptosis activity mediated by the crude pigment extract Y. The compounds in the crude pigment extract Y was identified by HR-LCMS analysis. Further studies on the active compounds can lead to a rise in new drugs for cancer treatment and also against rising antibacterial resistant pathogens.

中文翻译：

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内生菌伯克霍尔德菌色素的抗菌潜力和细胞凋亡诱导。WYAT7

来自内生菌伯克霍尔德菌的色素。WYAT7 从药用植物 Artemisia nilagirica (Clarke) Pamp 中分离出来。被提取。粗色素 Y 的抗菌功效被评估为抗革兰氏阳性和革兰氏阴性细菌病原体的抗生素来源。色素 Y 对测试的病原体伤寒杆菌 (MTCC 733)、金黄色葡萄球菌 (MTCC 1430)、铜绿假单胞菌 (MTCC 2453)、肺炎克雷伯菌 (MTCC 432)、大肠杆菌(MTCC 1610) 表现出显着的抗菌活性副伤寒 (3220)、枯草芽孢杆菌 (441) 和鲍曼不动杆菌 (12,889)。对大多数受试细菌病原体的粗色素提取物Y的最低抑菌浓度低于或等于0.25 µg/mL，最低杀菌浓度低于或等于0.5 µg/mL。在细胞毒性评估中，粗色素 Y 对正常细胞系 (L929) 表现出较小的毒性。粗色素提取物 Y 还对黑色素瘤癌细胞 (A375) 显示出强大的抗癌活性。获得的 IC50 值为 68.08 µg/mL。用色素处理的细胞的吖啶橙 (AO) 和溴化乙锭 (EB) 双染色有助于核凝聚、凋亡小体和活细胞的形态学评估。色素处理的 A375 细胞中的 DNA 片段分析和 caspase-9 定量测定了由色素粗提物 Y 介导的细胞凋亡活性。色素粗提物 Y 中的化合物通过 HR-LCMS 分析进行鉴定。对活性化合物的进一步研究可能会导致用于癌症治疗的新药增加，也可以对抗不断增加的抗细菌病原体。