Abstract It has been reported that high-mobility group box 3 is overexpressed in various cancers. This study aimed to explore its function in non-small cell lung cancer (NSCLC). A546 and H460 cell lines were used for in vivo experiments, scratch healing tests, transwell migration and invasion experiments. It was first found that HMGB3 was highly expressed in tumor tissues in the patients and associated with NSCLC stage. Silencing of HMGB3 significantly slowed the growth, proliferation and invasion of NSCLC in vitro, and repressed cell growth in vivo. Mechanistic studies suggest that the observed effects were mediated by inhibiting the expression of β-catenin/MMP7/c-Myc in Wnt pathway. Our study highlights the role of HMGB3 in NSCLC, which may provide a therapeutic target for the treatment of NSCLC.

中文翻译：

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高迁移率组盒 3 (HMGB3) 沉默通过调节 Wnt/β-catenin 通路抑制非小细胞肺癌的发展

摘要 据报道，高迁移率族框 3 在各种癌症中过表达。本研究旨在探讨其在非小细胞肺癌（NSCLC）中的作用。A546 和 H460 细胞系用于体内实验、划痕愈合测试、transwell 迁移和侵袭实验。首次发现HMGB3在患者肿瘤组织中高表达并与NSCLC分期相关。HMGB3的沉默在体外显着减缓了NSCLC的生长、增殖和侵袭，并在体内抑制了细胞生长。机制研究表明，观察到的作用是通过抑制 Wnt 通路中 β-catenin/MMP7/c-Myc 的表达来介导的。我们的研究强调了 HMGB3 在 NSCLC 中的作用，这可能为 NSCLC 的治疗提供治疗靶点。