Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease affecting the joints and surrounding tissue. Identification of novel proteins associated with the progression of a disease is a prerequisite for understanding the pathogenesis of RA. The present study was undertaken to identify the potential biomarkers from a less explored biological sample such as synovial fluid (SF) cells which is specific for RA and to analyze their functional aspects using proteomic approach. Two-dimensional gel electrophoresis (2-DE) was performed using synovial fluid cells of RA and osteoarthritis (OA) patients, and 7 differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS). Αlpha-Taxilin (α-Taxilin) has been found as one of the novel, significantly up regulated protein in RA. It has been validated in the synovium, synovial fluid (SF), SF cells, and plasma samples by Western blot, enzyme-linked immunosorbent assay (ELISA), fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and real-time PCR. The identification of autoantibody against α-Taxilin and in silico studies has further helped us to understand its involvement in disease mechanism. The present study will therefore provide knowledge towards the etiology of RA that pave the way for suitable prognostic marker identification along with other clinical parameters.

中文翻译：

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滑膜液细胞蛋白质组学分析可确定类风湿关节炎中α-塔西林蛋白的上调：一种潜在的预后标志物。

类风湿关节炎（RA）是一种慢性自身免疫性炎性疾病，会影响关节和周围组织。鉴定与疾病进展相关的新型蛋白质是了解RA发病机制的先决条件。进行本研究以从较少探索的生物学样品（例如对RA特有的滑液（SF）细胞）中识别潜在的生物标志物，并使用蛋白质组学方法分析其功能方面。使用RA和骨关节炎（OA）患者的滑液细胞进行二维凝胶电泳（2-DE），并使用基质辅助激光解吸/电离飞行时间质谱（MALDI- TOF MS / MS）。α-酪蛋白（α-Taxilin）被发现是RA中一种新颖的，上调的蛋白。已通过Western印迹，酶联免疫吸附测定（ELISA），荧光激活细胞分选（FACS），免疫组化（IHC）和真实-滑膜，滑液（SF），SF细胞和血浆样品对其进行了验证时间PCR。抗α- Taxilin自身抗体的鉴定和计算机研究进一步帮助我们了解其参与疾病机制的过程。因此，本研究将提供有关RA病因的知识，为适当的预后标志物鉴定以及其他临床参数铺平道路。