During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosum sensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.

中文翻译：

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从伊朗发现一种新品种的海棉复合体和海棉属的拮抗活性。在双重培养中抗灰烬病病原体Hymenoscyphus fraxineus。

在对伊朗北部的木瓜属真菌进行的一次调查中，收集并培养了一些标本，这些标本显示出与Hypoxylon rubiginosum复合物的亲和力。比较它们的形态特征，结合基于高效液相色谱的化学分类学研究，二极管阵列检测和质谱（HPLC-DAD / MS）以及基于ITS，LSU，rbp2和tub2 DNA的多位系统发育序列，揭示了这里描述的一种新物种，称为Hypoxylon guilanense。此外，还遇到了红花茄。同时，在我们实验室的双重培养试验中，后一种菌种的内生分离物显示出对Ash Dieback病原体Hymenoscyphus fraxineus的强拮抗活性。因此，我们决定测试这种新的伊朗真菌对这种病原体的拮抗活性，以及​​其他与H. rubiginosum相关的Hypoxylon菌种的几种培养物。我们的结果表明，Hypoxylon spp的拮抗作用。反对赞美诗。水痘是广泛存在的，并且它们是由于在病原体存在下产生了抗真菌的phomopsidin衍生物。