It is well known that nonalcoholic fatty liver disease (NAFLD) is a common disease worldwide because of unhealthy changes in dietary habits. In this study, we determined the effects of Tenebrio molitor Linnaeus, 1758 extract (TML) and Allomyrina dichotoma Linnaeus, 1771 larvae extract (ADL) in cellular and animal models. In vitro, TML and ADL treatments did not cause cytotoxicity, but attenuated the accumulation of lipid in HepG2 cells induced by free fatty acids. In vivo, mice were orally treated with TML and ADL for 10 weeks during high-fat diet feeding. TML and ADL administration significantly reduced the weight of body, liver tissue, and adipose tissue. Serum lipid profiles, hepatic functional parameters, and glucose levels were ameliorated by TML and ADL. Moreover, TML and ADL suppressed increased lipogenesis and inflammation-related makers, and improved antioxidant enzyme activity. In liver tissue, the decreased lipid accumulation by administration of TML and ADL was observed using Oil Red O and Hematoxylin and Eosin staining. Therefore, we suggest that TML and ADL may be having a therapeutic potential and is used to develop a therapeutic agent for NAFLD.

中文翻译：

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昆虫提取物对非酒精性脂肪肝疾病的有益作用。

众所周知，由于饮食习惯的不健康变化，非酒精性脂肪性肝病（NAFLD）是全世界范围内的常见疾病。在这项研究中，我们确定的影响黄粉虫林奈，1758提取物（TML）和Allomyrina萁林奈，1771幼虫提取物（ADL）在细胞和动物模型。在体外，TML和ADL处理并未引起细胞毒性，但减弱了由游离脂肪酸诱导的HepG2细胞中脂质的积累。体内在高脂饮食喂养期间，小鼠接受TML和ADL口服治疗10周。TML和ADL的使用显着减轻了人体，肝脏组织和脂肪组织的重量。TML和ADL改善了血清脂质谱，肝功能参数和葡萄糖水平。此外，TML和ADL抑制了脂肪形成和炎症相关物质的增加，并提高了抗氧化酶的活性。在肝组织中，使用油红O和苏木精和曙红染色观察到通过施用TML和ADL减少的脂质蓄积。因此，我们建议TML和ADL可能具有治疗潜力，并被用于开发NAFLD的治疗剂。