As a subtype of non-small-cell lung cancer, lung squamous cell carcinoma (LUSC) accounts for one-fifth of all lung cancers. Unfortunately, no specific targetable aberration has yet been identified. Hence, it is of huge urgency and potential to identify aberrantly regulated genes in LUSC. Here, five pairs of LUSC samples and their corresponding adjacent tissues were subject to whole transcriptome sequencing. Our results showed that CTD-2562J17.6 and FENDRR were significantly downregulated while MIR205HG, LNC_000378, RP11-116G8.5, RP3-523K23.2, and RP5-968D22.1 were significantly upregulated in all five LUSC samples. Importantly, MIR205HG was upregulated in LUSC clinical samples as well as in LUSC cell lines. Interestingly, our results demonstrated that the expression level of MIR205HG is positively correlated with the malignancy. In addition, MIR205HG is required for LUSC cell growth and cell migration. Most importantly, our results showed that MIR205HG prohibits LUSC apoptosis via regulating Bcl-2 and Bax. Taken together, our data shed lights on the lncRNA regulatory nexus that controls the carcinogenesis of LUSC and provided potential novel diagnostic markers and therapeutic targets for LUSC.

中文翻译：

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MIR205HG通过长期的非编码RNA分析揭示了体外促进肺鳞癌的癌变的能力。

作为非小细胞肺癌的一种亚型，肺鳞状细胞癌（LUSC）占所有肺癌的五分之一。不幸的是，尚未确定具体的目标像差。因此，鉴定LUSC中异常调节的基因具有巨大的紧迫性和潜力。在这里，对五对LUSC样品及其对应的相邻组织进行了完整的转录组测序。我们的结果表明，在所有五个LUSC样品中，CTD-2562J17.6和FENDRR均显着下调，而MIR205HG，LNC_000378，RP11-116G8.5，RP3-523K23.2和RP5-968D22.1均显着上调。重要的是，LUSC临床样品以及LUSC细胞系中的MIR205HG均被上调。有趣的是，我们的结果表明MIR205HG的表达水平与恶性肿瘤呈正相关。此外，LUSC细胞生长和细胞迁移需要MIR205HG。最重要的是，我们的结果表明MIR205HG通过调节Bcl-2和Bax来阻止LUSC凋亡。综上所述，我们的数据揭示了控制LUSC致癌作用的lncRNA调节联系，并为LUSC提供了潜在的新型诊断标记和治疗靶标。