A highly sensitive high-performance liquid chromatography method has been developed using the pre-column fluorescent derivatization of daptomycin (DAP) through cyclization of the amino group of ornithine with 2,3-naphthalenedialdehyde. With the proposed method, the limits of detection and quantification of DAP in murine serum were 8 and 3 nmol/L, respectively, and the calibration curve was linear across the examined dynamic range from 8 nmol/L to 1 μmol/L (n = 8, r = 0.9986). This method is suitable for animal experiments examining the side effects of DAP therapy using mice as a simple method with quantification to the order of 10 nmol/L.

通过鸟氨酸氨基与 2,3-萘二醛环化达托霉素 (DAP) 的柱前荧光衍生化，开发了一种高灵敏度高效液相色谱方法。采用该方法，鼠血清中 DAP 的检测限和定量限分别为 8 nmol/L 和 3 nmol/L，校准曲线在 8 nmol/L 至 1 μmol/L 的检测动态范围内呈线性（n = 8，r = 0.9986）。该方法适用于用小鼠检查 DAP 治疗副作用的动物实验，方法简单，定量至 10 nmol/L 量级。