The cassava hornworm Erinnyis ello ello (Lepidoptera: Sphingidae) is an important pest in Brazil. This insect feeds on host plants of several species, especially Manihot esculenta (cassava) and Hevia brasiliensis (rubber tree). Cassava hornworm outbreaks are quite common in Brazil and can cause great impact over crop production. Granulare and polyhedral-shaped occlusion bodies (OBs) were observed in extracts of dead E. ello larvae from rubber-tree plantations by light and scanning electron microscopy (SEM), suggesting a mixed infection. The polyhedral-shaped OB surface revealed indentations that resemble those found in cypovirus polyhedra. After OB nucleic acid extraction followed by cDNA production and Illumina deep-sequencing analysis, the results confirmed for the presence of a putative novel cypovirus that carries ten segments and also a betabaculovirus (Erinnyis ello granulovirus, ErelGV). Phylogenetic analysis of the predicted segment 1-enconded RdRP showed that the new cypovirus isolate is closely related to a member of species Cypovirus 2, which was isolated from Inachis io (Lepidoptera: Nymphalidae). Therefore, we named this new isolate Erinnyis ello cypovirus 2 (ErelCPV-2). Genome in silico analyses showed that ErelCPV-2 segment 8 (S8) has a predicted amino acid identity of 35.82 % to a hypothetical protein of betabaculoviruses. This putative protein has a cGAMP-specific nuclease domain related to the poxvirus immune nucleases (poxins) from the 2′,3′-cGAMP-degrading enzyme family.

中文翻译：

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在杆状病毒共感染环境中发现的新型杯状病毒含有痘病毒免疫核酸酶（poxin）相关基因。

木薯角虫Erinnyis ello ello（鳞翅目：天蛾科）是巴西的重要害虫。这种昆虫以几种物种的寄主植物为食，尤其是Manihot esculenta（木薯）和Hevia brasiliensis（橡胶树）。木薯天牛爆发在巴西很普遍，可能对农作物产量产生巨大影响。在死去的大肠杆菌提取物中观察到颗粒状和多面体形的咬合体（OB）橡胶树人工林的幼虫通过光和扫描电子显微镜（SEM）观察，表明是混合感染。多面体形的OB表面显示出类似于在杯状病毒多面体中发现的压痕。OB核酸提取后，进行cDNA产生和Illumina深度测序分析，结果证实了存在推定的新型cypovirus，该携带10个片段，也包含β杆状病毒（Erinnyis ello粒状病毒，ErelGV）。系统进化分析预测的片段1包围的RdRP显示，新的杯状病毒分离株与从Inachis io（鳞翅目：N科）分离出的第二种杯状病毒2的成员密切相关。因此，我们将这种新的分离株命名为埃里尼索氏大肠杆菌病毒2（ErelCPV-2）。基因组在计算机分析中，ErelCPV-2区段8（S8）与β杆状病毒的假定蛋白的预测氨基酸同一性为35.82％。该推定蛋白具有与来自2'，3'-cGAMP降解酶家族的痘病毒免疫核酸酶（poxin）相关的cGAMP特异性核酸酶结构域。