Background: A reduced concentration of adiponectin is considered as an independent factor of the risk of inducing endometrial cancer. Cisplatin is a drug used in the therapy of this type of neoplasm. However, knowledge of the effects of cisplatin on the adiponectin level is still limited.

Objective: The purpose of this study was to assess the impact of cisplatin depending on the concentration and time of exposition of the cells to the drug on the adiponectin level in the endometrial cancer cell line.

Methods: Cells of endometrial cancer cell line Ishikawa were exposed for 12,24 and 48 hour periods to cisplatin with the following concentrations: 2.5μM, 5μM, 10μM. The changes in the expression profile of adiponectin were compared to the RtqPCR reaction and ELISA test. The STATISTICA 13.0 PL program was used for statistical analysis (p<0.05).

Results: In the culture without the drug, the concentration of adiponectin was statistically lower than in the cell culture incubated with the drug. Changes on the mRNA level seem to be more specific than on the protein level, although in both cases, the same trend in the expression changes was noted.

Discussion: The longer the time of exposition of the cells to the drug, the expression of mRNA, and the adiponectin protein increased. Changes in the expression profile were characterized statistically (p<0.05).

Conclusion: Cisplatin, in a noticeable way, changes the expression profile of adiponectin. Molecular analysis indicated that in the case of endometrial cancer therapy should be implemented with a concentration of no less than 5 μM.

背景：脂联素浓度降低被认为是诱发子宫内膜癌风险的独立因素。顺铂是用于治疗这类肿瘤的药物。但是，关于顺铂对脂联素水平影响的知识仍然有限。

目的：本研究的目的是评估顺铂对子宫内膜癌细胞株中脂联素水平的影响，具体取决于细胞暴露于药物的浓度和时间。

方法：将子宫内膜癌细胞系Ishikawa的细胞暴露于以下浓度的顺铂中12,24和48小时：2.5μM，5μM，10μM。将脂联素表达谱的变化与RtqPCR反应和ELISA测试进行比较。使用STATISTICA 13.0 PL程序进行统计分析（p <0.05）。

结果：在没有药物的培养物中，脂联素的浓度在统计学上低于与药物一起培养的细胞培养物中的浓度。尽管在这两种情况下，都注意到表达变化的相同趋势，但mRNA水平的变化似乎比蛋白质水平更特异性。

讨论：细胞暴露于药物的时间越长，mRNA的表达和脂联素蛋白增加。表达谱的变化具有统计学特征（p <0.05）。

结论：顺铂明显改变了脂联素的表达谱。分子分析表明，对于子宫内膜癌，应以不小于5μM的浓度进行治疗。