The compound 2,4-diacetylphloroglucinol (DAPG) is a well-known secondary metabolite produced by Pseudomonas spp. that are used as biocontrol agents. DAPG displays a remarkably broad spectrum of toxic activity against pathogens of plants. Yet high concentrations of DAPG may also have negative effect on plants, but the phytotoxicity of DAPG is not clearly understood. Here, we used genome-wide activation, tagging Arabidopsis plants as the model plant to investigate the plant response to DAPG. A total of 15 lines were selected as DAPG-tolerant plants from among 62,000 lines investigated. The DAPG-responsible genes were then identified via thermal asymmetric interlaced PCR and quantitative reverse transcription PCR, and the gene ontology analysis showed the distribution of these genes having different biological processes, cellular regulations, and molecular functional properties. Collectively, these findings suggest that plants may rely on several pathways to prevent DAPG phytotoxicity.

化合物2,4-二乙酰基间苯三酚（DAPG）是假单胞菌属（Pseudomonas spp）产生的众所周知的次级代谢产物。用作生物防治剂。DAPG对植物病原体显示出广泛的毒性。然而，高浓度的DAPG可能对植物也有负面影响，但尚不清楚DAPG的植物毒性。在这里，我们使用了全基因组激活，标记了拟南芥以植物为模型植物，以研究植物对DAPG的反应。从研究的62,000个品系中，总共选择了15个品系作为耐DAPG的植物。然后通过热不对称隔行PCR和定量逆转录PCR鉴定出负责DAPG的基因，基因本体分析表明这些基因的分布具有不同的生物学过程，细胞调控和分子功能特性。总而言之，这些发现表明植物可能依赖于几种途径来预防DAPG的植物毒性。