The poly(ADP-ribose) polymerase 1 (PARP-1) enzyme has received much attention in the last decade due to its promising role in cancer therapeutics. Despite the expanding use of PARP inhibitors in cancer therapy, little is known about PARP-1 tissue distribution. Our study provides a detailed survey of PARP-1 tissue and cellular distribution using well-preserved cynomolgus monkey organs and a well-characterized, highly specific monoclonal PARP-1 antibody. Overall, PARP-1 was detected in most organs, but its distribution was restricted to specific cells within each tissue, suggesting that PARP-1 expression is tightly regulated. The strongest expression was in the pituitary, the ovary, the male adrenal gland, and the thymus. One of the key findings of this study was the stronger expression of PARP-1 in proliferating cells rather than mature cells. This observation not only provides clues to the importance of PARP-1 in processes such as DNA replication and transcription in these cell types, but it also provides the basis for further investigation into the effects of its inhibition in the context of malignancy. Overall, this study greatly expands the current knowledge of PARP-1 tissue expression, enabling the identification of tissues where PARP inhibition may be most efficacious.

中文翻译：

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食蟹猴组织中PARP-1分布的评估。

聚（ADP-核糖）聚合酶1（PARP-1）酶由于在癌症治疗中有希望的作用而在最近十年受到了广泛关注。尽管PARP抑制剂在癌症治疗中的使用越来越广泛，但对PARP-1组织分布的了解却很少。我们的研究使用保存完好的食蟹猴器官和特征明确的高度特异性单克隆PARP-1抗体对PARP-1组织和细胞分布进行了详细调查。总体而言，在大多数器官中都检测到PARP-1，但其分布仅限于每个组织内的特定细胞，这表明PARP-1的表达受到严格调节。表达最强的是垂体，卵巢，雄性肾上腺和胸腺。这项研究的主要发现之一是PARP-1在增殖细胞而不是成熟细胞中的更强表达。该观察不仅提供了PARP-1在这些细胞类型中的DNA复制和转录等过程中的重要性的线索，而且还为进一步研究其在恶性肿瘤中的抑制作用提供了基础。总体而言，这项研究极大地扩展了PARP-1组织表达的当前知识，从而能够鉴定出对PARP-1抑制作用最有效的组织。