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Larvicidal activity of Azadirachta indica, Melaleuca alternifolia, and Carapa guianensis oil compounds and Carica papaya fermented extract on Aedes aegypti
bioRxiv - Systems Biology Pub Date : 2020-08-10 , DOI: 10.1101/2020.05.18.102442
Sandra M. Torres , Luis A.R. Lima , Maria do Carmo A. Lima , Lêucio C. Alves , Valdemiro A.S. Júnior

The aim of this study was to evaluate the structural changes of Aedes aegypti larvae submitted to treatment with a product based on Azadirachta indica, Melaleuca alternifolia, Carapa guianensis oils and Carica papaya fermented extract. The larvae were exposed for 24h to the experimental groups: product formulated in concentrations of 50% (G1), 25% (G2), and 12.5% (G3); and negative control groups based on water (CN1) and dimethyl sulfoxide (CN2). By the end of the experimental period, some larvae were fixed in 4% buffered glutaraldehyde solution to be processed for optical microscopy. Larvae exposed to G2 and G3 presented more structural damage of the mesentery, Malpighi tubules and nerve ganglia. We conclude that the product formulated in 12.5% and 25% concentrations can be used in the population control of the 3rd larvae stage of Aedes aegypti by causing lethal injuries and avoiding the larvae development.

中文翻译:

印A,白千层和圭亚那Car油化合物和番木瓜发酵提取物对埃及伊蚊的杀幼虫活性

这项研究的目的是评估埃及伊蚊幼虫的结构变化,该结构需使用基于印度印za,千层白千层,Carapa guianensis油和番木瓜发酵提取物的产品进行处理。将幼虫暴露于实验组24小时:以50%(G1),25%(G2)和12.5%(G3)的浓度配制产品;以及基于水(CN1)和二甲基亚砜(CN2)的阴性对照组。实验结束时,将一些幼虫固定在4%的戊二醛缓冲液中,进行光学显微镜处理。暴露于G2和G3的幼虫对肠系膜,Malpighi小管和神经节的结构损害更大。我们得出结论,该产品配方为12。
更新日期:2020-08-11
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