Recent advances in optical microscopy instrumentation and processing techniques have led to imaging that both breaks the diffraction barrier and enables sub-pixel resolution. This enhanced resolution has expanded the capabilities of particle tracking to nanoscale processes in soft matter including biomolecular, colloidal, and polymeric materials. This tutorial provides a basic understanding of particle tracking instrumentation, the fundamentals of tracking analysis, and potential sources of error and bias inherent in analyzing particle tracking. Finally, we provide a brief outlook for the future of particle tracking through the lens of machine learning.

中文翻译：

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软物质中纳米粒子的粒子追踪

光学显微镜仪器和处理技术的最新进展使成像既打破了衍射障碍，又实现了亚像素分辨率。这种增强的分辨率将粒子跟踪的能力扩展到软物质中的纳米级过程，包括生物分子、胶体和聚合物材料。本教程提供了对粒子跟踪仪器、跟踪分析的基本原理以及分析粒子跟踪中固有的潜在误差和偏差来源的基本了解。最后，我们通过机器学习的视角对粒子跟踪的未来进行了简要展望。