Abstract

Nonalcoholic fatty liver disease (NAFLD) and obesity are becoming increasingly common globally and characteristic as gut microbiota disturbance. Supplement of probiotics is considered as a promising strategy for NAFLD and obesity treatment. However, this effect varied from each other in clinical trials. We proposed that combination with a prebiotic substrate may improve the effects of probiotics. Thus, in this study, we investigated the separated and combined effects of Bifidobacteria and resveratrol (RSV) against obesity and NAFLD. NAFLD was caused by high-fat diet (HFD) feeding for 8 weeks. HFD-treated mice were orally treated with B. longum (1 × 10⁹ CFU/mouse/day), RSV (100 mg/kg/day), and both of them from the fifth week. HFD feeding caused obesity and NAFLD as indicated by significantly increased body and liver weights, liver steatosis, elevated serum transaminases and lipid profiles, increased inflammation and imbalanced redox status. Based on these physical and biochemical parameters, inflammatory and antioxidant markers, individual administration of B. longum and RSV alleviated obesity and NAFLD, while coadministration of both products further enhanced the efficacy. These data suggested that combined prebiotic RSV and probiotic B. longum would be a potential candidate or adjuvant for the treatment of obesity and NAFLD.

摘要

非酒精性脂肪性肝病 (NAFLD) 和肥胖症在全球范围内变得越来越普遍，并且以肠道微生物群紊乱为特征。补充益生菌被认为是治疗 NAFLD 和肥胖症的一种有前景的策略。然而，这种效果在临床试验中各不相同。我们提出与益生元底物的组合可以改善益生菌的作用。因此，在本研究中，我们研究了双歧杆菌和白藜芦醇 (RSV) 对肥胖和 NAFLD的分离和联合作用。NAFLD 是由高脂肪饮食 (HFD) 喂养 8 周引起的。用长双歧杆菌(1 × 10 ⁹CFU/小鼠/天）、RSV（100 毫克/千克/天），以及第 5 周的两者。HFD 喂养导致肥胖和 NAFLD，表现为体重和肝脏重量显着增加、肝脏脂肪变性、血清转氨酶和脂质谱升高、炎症增加和氧化还原状态不平衡。基于这些物理和生化参数、炎症和抗氧化标志物，长双歧杆菌和 RSV 的单独给药减轻了肥胖和 NAFLD，同时两种产品的共同给药进一步增强了疗效。这些数据表明，益生元 RSV 和益生菌长双歧杆菌的组合将成为治疗肥胖症和 NAFLD 的潜在候选者或佐剂。