Pancreatic cancer (PC), highly malignant, is one of the most lethal cancers. Interferon-induced transmembrane protein 1 (IFITM1) has recently been regarded as a new molecular marker in human cancers. However, the role of IFITM1 in PC remains unclear. In this study, a short hairpin RNA (shRNA) was constructed to assess the effect of IFITM1 on PANC-1 and ASPC-1 cells. The level of IFITM1 was downregulated in cells transfected with shRNA targeting IFITM1 (sh-IFITM1). Silencing of IFITM1 significantly decreased cell viability, downregulated the level of Ki-67, arrested cell at G1/S phase, reduced the number of cells in S phase, and decreased cyclinD1, cyclinE, CDK2, and CDK4 levels. Moreover, Hoechst staining and Western blotting analysis showed that cell apoptosis was induced by IFITM1. IFITM1 knockdown suppressed the MAPK signaling pathway by downregulation of p-ERK, p-P38, and p-JNK levels. These findings suggested that IFITM1 could be considered a potential therapeutic target for PC.

高度恶性的胰腺癌（PC）是最致命的癌症之一。干扰素诱导的跨膜蛋白1（IFITM1）最近被认为是人类癌症中的新分子标记。但是，IFITM1在PC中的作用仍不清楚。在这项研究中，构建了一个短发夹RNA（shRNA），以评估IFITM1对PANC-1和ASPC-1细胞的作用。在用靶向IFITM1（sh-IFITM1）的shRNA转染的细胞中，IFITM1的水平下调。沉默IFITM1会显着降低细胞活力，下调Ki-67水平，将细胞停在G1 / S期，减少S期细胞数量并降低cyclinD1，cyclinE，CDK2和CDK4水平。此外，Hoechst染色和蛋白质印迹分析表明，IFITM1诱导了细胞凋亡。IFITM1抑制通过下调p-ERK，p-P38和p-JNK水平抑制MAPK信号通路。这些发现表明，IFITM1被认为是PC的潜在治疗靶标。