Xylooligosaccharides (XOS) are attracting an ever-increasing amount of interest for use as food prebiotics. In this study, we used efficient membrane separation technology to convert lignocellulosic materials into a renewable source of XOS. This study revealed a dual function of nanofiltration membranes by first achieving a high yield of xylobiose (a main component of XOS) from alkali-pretreated empty fruit bunch (EFB) hydrolysate, and then by achieving a high degree of separation for xylose as a monosaccharide product. Alkali pretreatment could increase the xylan content retention of raw EFB from 23.4% to 26.9%, which eventually contributed to higher yields of both xylobiose and xylose. Nanofiltration increased the total amount of XYN10Ks_480 endoxylanase produced from recombinant Streptomyces lividans 1326 without altering its specific activity. Concentrated XYN10Ks_480 endoxylanase was applied to the recovery of both xylobiose and xylose from alkali-pretreated EFB hydrolysate. Xylobiose and xylose yields reached 41.1% and 17.3%, respectively, and when unconcentrated XYN10Ks_480 endoxylanase was applied, those yields reached 35.1% and 8.3%, respectively. The last step in nanofiltration was to separate xylobiose over xylose, and 41.3 g.L⁻¹ xylobiose (90.1% purity over xylose) was achieved. This nanofiltration method should shorten the processes used to obtain XOS as a high-value end product from lignocellulosic biomass.

中文翻译：

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通过空滤从空果束中高酶回收和纯化木寡糖

木寡糖（XOS）越来越多地被用作食品益生元。在这项研究中，我们使用了高效的膜分离技术将木质纤维素材料转化为XOS的可再生资源。这项研究揭示了纳米过滤膜的双重功能：首先从碱预处理的空果束（EFB）水解物中获得高产率的木糖（XOS的主要成分），然后实现木糖作为单糖的高度分离产品。碱预处理可以将原始EFB的木聚糖含量保留率从23.4％提高到26.9％，最终有助于提高木二糖和木糖的产量。纳滤增加了重组猪链霉菌产生的XYN10Ks_480内切木聚糖酶的总量1326年没有改变其具体活动。将浓缩的XYN10Ks_480内切木聚糖酶用于从碱预处理的EFB水解产物中回收木糖和木糖。木糖和木糖的产率分别达到41.1％和17.3％，并且当应用未浓缩的XYN10Ks_480内切木聚糖酶时，那些产率分别达到35.1％和8.3％。纳滤的最后一步是将木糖与木糖分离，从而获得41.3 gL ^-1的木糖（纯度比木糖高90.1％）。这种纳滤方法应缩短从木质纤维素生物质获得高价值最终产品XOS的过程。