Poly(methyl-methacrylate) (PMMA) is a biocompatible and non-biodegradable polymer widely used as biomedical material. PMMA microcapsules with suitable dimension and porosity range are proposed to encapsulate live cells useful for tissue regeneration purposes. The aim of this work was to evaluate the feasibility of producing cell-loaded PMMA microcapsules through “high efficiency vibrational technology” (HEVT). Preliminary studies were conducted to set up the process parameters for PMMA microcapsules production and human dermal fibroblast, used as cell model, were encapsulated in shell/core microcapsules. Microcapsules morphometric analysis through optical microscope and scanning electron microscopy highlighted that uniform microcapsules of 1.2 mm with circular surface pores were obtained by HEVT. Best process conditions used were as follows: frequency of 200 Hz, voltage of 750 V, flow rate of core solution of 10 mL/min, and flow rate of shell solution of 0.5 bar. Microcapsule membrane allowed permeation of molecules with low and medium molecular weight up to 5900 Da and prevented diffusion of high molecular weight molecules (11,000 Da). The yield of the process was about 50% and cell encapsulation efficiency was 27% on total amount. The cell survived and growth up to 72 h incubation in simulated physiologic medium was observed.

中文翻译：

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用于聚合物微胶囊中细胞封装的高效振动技术（HEVT）。

聚（甲基丙烯酸甲酯）（PMMA）是一种生物相容且不可生物降解的聚合物，被广泛用作生物医学材料。提出了具有合适的尺寸和孔隙率范围的PMMA微囊来包封用于组织再生目的的活细胞。这项工作的目的是评估通过“高效振动技术”（HEVT）生产细胞负载的PMMA微胶囊的可行性。进行了初步研究以建立用于生产PMMA微囊的工艺参数，并将用作细胞模型的人真皮成纤维细胞封装在壳/核微囊中。通过光学显微镜和扫描电子显微镜对微胶囊的形态分析表明，通过HEVT获得了具有圆形表面孔的1.2mm的均匀微胶囊。最佳处理条件如下：频率为200 Hz，电压为750 V，核心溶液流量为10 mL / min，壳溶液流量为0.5 bar。微胶囊膜允许低至中等分子量至5900 Da的分子渗透，并阻止高分子量分子（11,000 Da）扩散。该方法的产率为约50％，并且细胞包封效率为总量的27％。细胞存活并观察到在模拟生理培养基中温育长达72小时的生长。该方法的产率为约50％，并且细胞包封效率为总量的27％。细胞存活并观察到在模拟生理培养基中温育长达72小时的生长。该方法的产率为约50％，并且细胞包封效率为总量的27％。细胞存活并观察到在模拟生理培养基中温育长达72小时的生长。