Antibiotic‐resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF‐P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF‐P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF‐P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF‐P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5‐aminopentanol modification of S. aureus EF‐P.

中文翻译：

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来自金黄色葡萄球菌的延伸因子 P 的翻译后修饰。

耐抗生素金黄色葡萄球菌正在成为许多国家卫生保健系统的主要负担，需要确定抗生素开发的新目标。延伸因子 P (EF-P) 是一种高度保守的延伸蛋白因子，在蛋白质合成和细菌毒力中起重要作用。EF-P正确地进行以逐步的方式发挥功能的独特的翻译后修饰，但对EF-P的翻译后修饰的实验信息是目前所缺乏用于小号。 金黄色葡萄球菌。在这里，我们用S表示 EF-P 。 金黄色葡萄球菌通过质谱分析其翻译后修饰并报告 5-氨基戊醇修饰的实验证据小号。 金黄色葡萄球菌EF-P。