Odorant binding proteins (OBPs) are a group of soluble proteins functioning as odorant carriers in insect antennae, mouth parts and other chemosensory organs. However, multiple insect OBPs have been detected in other tissues and various functions have been proposed. Therefore, a detailed expression profile including stages, tissues and sexes where OBPs are expressed will assist in building the links to their potential functions, enhancing the functional studies of insect OBPs. Here, we identified 39 putative OBP genes from its genome and transcriptome sequences of diamondback moth (DBM), Plutella xylostella. The expression patterns of identified PxylOBPs were further investigated from eggs, larvae, pupae, virgin adults, mated adults, larval midgut, larval heads, adult antennae, adult heads and adult tarsi. Moreover, P. xylostella larvae and adults with and without host plants for 5 h were utilized to study the interactions between OBP expression and host plants. The results showed that most PxylOBPs were highly expressed in male and female adult antennae. The expression levels of certain PxyOBPs could be regulated by mating activities and feeding host plants. This study advances our knowledge of P. xylostella OBPs, which may help develop new strategies for more environmentally sustainable management of P. xylostella.

中文翻译：

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小菜蛾 Plutella xylostella 中气味结合蛋白的鉴定和表征

气味结合蛋白 (OBP) 是一组可溶性蛋白质，在昆虫触角、口部和其他化学感应器官中充当气味载体。然而，已经在其他组织中检测到多种昆虫 OBP，并提出了各种功能。因此，包括表达 OBP 的阶段、组织和性别的详细表达谱将有助于建立与其潜在功能的联系，加强昆虫 OBP 的功能研究。在这里，我们从小菜蛾 (DBM)、小菜蛾( Plutella xylostella ) 的基因组和转录组序列中鉴定了 39 个假定的 OBP 基因. 从卵、幼虫、蛹、处女成虫、交配成虫、幼虫中肠、幼虫头部、成虫触角、成虫头部和成虫跗骨中进一步研究了鉴定的 PxylOBP 的表达模式。此外，小菜蛾幼虫和成虫有和没有寄主植物 5 小时被用来研究 OBP 表达和寄主植物之间的相互作用。结果表明，大多数PxylOBPs在雄性和雌性成虫触角中高度表达。某些 PxyOBPs 的表达水平可以通过交配活动和取食寄主植物来调节。这项研究增进了我们对小菜蛾OBP 的了解，这可能有助于制定新的策略，以更环保地可持续管理小菜蛾。