Break Up to Make Up: Utilization of Photocleavable Groups in Biolabeling of Hydrogel Scaffolds,ChemPhotoChem

当前位置： X-MOL 学术 › ChemPhotoChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Break Up to Make Up: Utilization of Photocleavable Groups in Biolabeling of Hydrogel Scaffolds
ChemPhotoChem ( IF 3.0 ) Pub Date : 2020-06-17 , DOI: 10.1002/cptc.202000072
Vinh X. Truong _{1,

2}

Affiliation

Understanding cell behavior within the extracellular matrix is essential for the implementation of biomaterials in medical applications. Conjugation of bioactive molecules to hydrogel scaffolds imparts the materials with biological cues necessary for controlling cell behavior such as adhesion, growth and migration. Photo‐induced ligation is a powerful tool to precisely control when and where such bioactivity can be initiated. Over the past decade, advances in light‐regulated technology have emerged to address the stringent demands for photochemical ligation in biological environments, including high sensitivity, fast reaction kinetics, longer wavelength absorption and site‐selectivity. Here, the utilization of contemporary photolabile groups for light‐mediated bioconjugation of hydrogels are highlighted. In this approach, reactive groups such as thiol, amine and aldehyde are masked with photolabile moieties, which can be photo‐released to participate in click reactions, such as Michael addition and oxime ligation. New chromophores with long wavelength visible light absorption are also examined for potential employment in light‐induced bioconjugation.

中文翻译：

分手弥补：水凝胶支架生物标记中光裂解基团的利用。

了解细胞外基质内的细胞行为对于在医疗应用中实施生物材料至关重要。生物活性分子与水凝胶支架的结合为材料赋予了控制细胞行为（如粘附，生长和迁移）所必需的生物学线索。光诱导的结扎是精确控制何时何地可以启动这种生物活性的强大工具。在过去的十年中，为了满足生物环境中光化学连接的严格要求，光调节技术已经出现，包括高灵敏度，快速反应动力学，更长的波长吸收和位点选择性。在这里，强调了当代光不稳定基团在光介导的水凝胶生物结合中的利用。用这种方法诸如硫醇，胺和醛等反应性基团被光不稳定部分掩盖，这些部分可被光释放以参与点击反应，例如迈克尔加成和肟连接。还研究了具有长波长可见光吸收的新生色团在光诱导生物共轭中的潜在用途。

更新日期：2020-06-17

点击分享查看原文

点击收藏

阅读更多本刊最新论文