Microbial production of 4-amino-1-butanol, a four-carbon amino alcohol.,Biotechnology and Bioengineering

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Microbial production of 4-amino-1-butanol, a four-carbon amino alcohol.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-05-21 , DOI: 10.1002/bit.27438
Cindy Pricilia Surya Prabowo _{1,

2} , Jae Ho Shin _{1,

2} , Jae Sung Cho _{1,

2} , Tong Un Chae _{1,

2} , Sang Yup Lee _{1,

2,

3}

Affiliation

4‐Amino‐1‐butanol (4AB) serves as an important intermediate compound for drugs and a precursor of biodegradable polymers used for gene delivery. Here, we report for the first time the fermentative production of 4AB from glucose by metabolically engineered Corynebacterium glutamicum harboring a newly designed pathway comprising a putrescine (PUT) aminotransferase (encoded by ygjG) and an aldehyde dehydrogenase (encoded by yqhD) from Escherichia coli, which convert PUT to 4AB. Application of several metabolic engineering strategies such as fine‐tuning the expression levels of ygjG and yqhD, eliminating competing pathways, and optimizing culture condition further improved 4AB production. Fed‐batch culture of the final metabolically engineered C. glutamicum strain produced 24.7 g/L of 4AB. The strategies reported here should be useful for the microbial production of primary amino alcohols from renewable resources.

中文翻译：

4-氨基-1-丁醇（一种四碳氨基醇）的微生物生产。

4-氨基-1-丁醇（4AB）是重要的药物中间体和用于基因传递的可生物降解聚合物的前体。在这里，我们首次报道了代谢工程谷氨酸棒杆菌从葡萄糖发酵生产 4AB，该谷氨酸棒杆菌包含一条新设计的途径，包括腐胺 (PUT) 转氨酶（由ygjG编码）和来自大肠杆菌的醛脱氢酶（由yqhD 编码），将 PUT 转换为 4AB。几种代谢工程策略的应用，例如微调ygjG和yqhD的表达水平、消除竞争途径和优化培养条件进一步提高了 4AB 产量。最终代谢工程谷氨酸棒杆菌菌株的补料分批培养产生 24.7 g/L 的 4AB。这里报告的策略应该对从可再生资源微生物生产伯氨基醇有用。

更新日期：2020-05-21

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