In this study, dogs with atopic dermatitis were separated into non-food-induced atopic dermatitis (NFIAD) group (n = 15) and food-induced atopic dermatitis (FIAD) group (n = 37) based on an elimination diet test. IgE reactivity for crude Malassezia pachydermatis (M. pachydermatis) and house dust mites (HDM) allergen extracts was investigated in the two groups using fluorometric enzyme-linked immunosorbent assay (ELISA) and intradermal skin test (IDST). Nine (60%) of the 15 dogs in NFIAD group and 6 (16%) of the 37 dogs in FIAD group showed specific IgE for M. pachydermatis (Mann-Whitney U-test, P < 0.01). By immunoblotting analysis, the pooled serum samples from dogs with IgE for M. pachydermatis showed IgE reactivity for 50 kDa protein of M. pachydermatis. Twelve (80%) of the 15 dogs in NFIAD group and 8 (22%) of the 37 dogs in FIAD group showed specific IgE for HDM (Mann-Whitney U-test, P < 0.01). In addition, the dogs in NFIAD group significantly show a positive IDST to M. pachydermatis and HDM extracts compared with the dogs in FIAD group. The results suggest that dogs with NFIAD are at increased risk of becoming sensitized to the normal commensal organism M. pachydermatis compared with dogs with FIAD, perhaps co-sensitization occurred due to an HDM protease antigen’s, Der f 1 and/or Der p 1, proteolytic activity related epidermal skin barrier defects. Treatment to limit skin colonization may thus be especially important in NFIAD.

在这项研究中，根据消除饮食测试，将患有特应性皮炎的狗分为非食物诱发性特应性皮炎（NFIAD）组（n = 15）和食物诱发性特应性皮炎（FIAD）组（n = 37）。使用荧光酶联免疫吸附试验（ELISA）和皮内皮肤试验（IDST），研究了两组中粗制的马拉色霉菌（M. pachydermatis）和屋尘螨（HDM）过敏原提取物的IgE反应性。在15只狗在37只犬FIAD组NFIAD组和6（16％）的九（60％）显示出特异性IgE为M. pachydermatis（曼-惠特尼U检验，P <0.01）。通过免疫印迹分析，从犬中分离出具有IgE的犬血清型支原体帕金森氏菌对厚皮支原体50 kDa蛋白有IgE反应性。NFIAD组的15只狗中有十二只（80％），FIAD组的37只狗中有8只（22％）对HDM表现出特异性IgE（Mann-Whitney U检验，P <0.01）。此外，在NFIAD组狗显著表现出积极的IDST到M. pachydermatis并与FIAD组的狗相比，HDM提取物。结果表明，犬NFIAD有成为敏化到正常共生生物风险增加M. pachydermatis与犬FIAD相比，或许共增感发生由于HDM酶抗原的，粉尘螨1和/或的Der p 1，蛋白水解活性与表皮皮肤屏障缺陷有关。因此，限制皮肤定植的治疗在NFIAD中尤其重要。