The design and synthesis of high selective and sensitive fluorescence chemosensor for the determination of ClO⁻ has received great efforts due to its critically important role in the immune system, however, most of chemosensor for detecting ClO⁻ by using single-channel fluorescent signal, which is susceptible to many factors. Herein, a fluorescence resonance energy transfer (FRET)-based ratiometric and colormetric chemosensor containing a 1,8-naphthalimide fluorophore and a rhodamine fluorophore is designed and synthesized for the detection of ClO⁻, and its structure is characterized by NMR and ESI-MS. The reaction between ClO⁻ and thiosemicarbazide causes the transform of the rhodamine fluorophore from the ring-closed form to the ring-opened form, showing a FRET process with the fluorescence color change from blue to orange. The chemosensor is successfully used for the quantitative detection of ClO⁻ based on the ratio between the fluorescence emission intensities of the two forms, and exhibits a wide linear range of 0–60 μM (R = 0.996) and the detection of limit as low as 4.50 nM. In addition, the detection is highly selective and not significantly affected by various reactive oxygen species (ROS), RNS and other anions in DMSO/PBS buffer (6/4, v/v). The chemosensor can be used for cell staining.

设计和高选择性和CLO的测定敏感的荧光化学传感器的合成^-已经由于其在免疫系统中，但是，大多数化学传感器，用于检测CLO极其重要的作用受到了极大的努力^-通过使用单信道的荧光信号，其容易受到许多因素的影响。在本文中，荧光共振能量转移（FRET）的含有1,8-萘二甲酰亚胺荧光团和荧光团罗丹明比例和比色化学传感器被设计并用于检测CLO的合成^- ，其结构的特征在于，通过NMR和ESI-MS 。CLO之间的反应^-硫代氨基脲会导致若丹明荧光团从闭环形式转变为开环形式，表明FRET过程的荧光颜色从蓝色变为橙色。该化学传感器被成功地用于CLO的定量检测^-基于所述两种形式的荧光发射强度之间的比率，并且表现出0-60μM（R = 0.996）和极限的检测低至线性范围宽4.50 nM。此外，该检测具有很高的选择性，并且不受DMSO / PBS缓冲液（6/4，v / v）中各种活性氧（ROS），RNS和其他阴离子的明显影响。化学传感器可用于细胞染色。