The bacterial reverse mutation test (Ames test) is the most commonly used genotoxicity test; it is a primary component of the chemical safety assessment data required by regulatory agencies worldwide. Within the current accepted in vitro genotoxicity test battery, it is considered capable of revealing DNA reactivity, and identifying substances that can produce gene mutations via different mechanisms. The previously published consolidated EURL ECVAM Genotoxicity and Carcinogenicity Database, which includes substances that elicited a positive response in the Ames test, constitutes a collection of data that serves as a reference for a number of regulatory activities in the area of genotoxicity testing. Consequently, we considered it important to expand the database to include substances that fail to elicit a positive response in the Ames test, i.e., Ames negative substances. Here, we describe a curated collection of 211 Ames negative substances, with a summary of complementary data available for other genotoxicity endpoints in vitro and in vivo, plus available carcinogenicity data. A descriptive analysis of the data is presented. This includes a representation of the chemical space formed by the Ames-negative database with respect to other substances (e.g. REACH registered substances, approved drugs, pesticides, etc.) and a description of the organic functional groups found in the database. We also provide some suggestions on further analyses that could be made.

细菌回复突变试验（Ames test）是最常用的基因毒性试验；它是全球监管机构要求的化学品安全评估数据的主要组成部分。在目前接受的体外基因毒性测试电池，它被认为能够揭示 DNA 反应性，并识别可通过不同机制产生基因突变的物质。先前发布的综合 EURL ECVAM 基因毒性和致癌性数据库，其中包括在艾姆斯试验中引起阳性反应的物质，构成了一组数据，可作为基因毒性试验领域许多监管活动的参考。因此，我们认为扩展数据库以包括在 Ames 测试中未能引起阳性反应的物质（即 Ames 阴性物质）很重要。在这里，我们描述了 211 种 Ames 阴性物质的精选集合，并总结了可用于其他体外和遗传毒性终点的补充数据。体内，加上可用的致癌性数据。对数据进行了描述性分析。这包括 Ames-negative 数据库形成的化学空间表示与其他物质（例如 REACH 注册物质、批准的药物、农药等）以及数据库中发现的有机官能团的描述。我们还就可以进行的进一步分析提供了一些建议。