BACKGROUND The Mycoplasma pneumoniae (M.pneumoniae) was accounted to 3-10% of total pneumonia incidences. In recent decades, metallic nanoparticles were extensively examined as nano-antibiotics. OBJECTIVE In this investigation, we intended to inspect the therapeutic potential of Zinc oxide nanoparticles (ZnONPs) from (Corydalis yanhusuo) C. yanhusuo against the mycoplasma infected pneumonia in mice. METHODOLOGY The ZnONPs were formulated via green route technique and characterized by UV-vis spectroscopy, transmission electron microscopy, Fourier transform infrared technique, and atomic force microscopy. The antimicrobial activity of formulated ZnONPs was tested by well diffusion method. The total protein, interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α) and transforming growth factor (TGF) status in the BALF of M. pneumonia infected animals were investigated via kit method. The expressions of ERK1/2, JNK1/2, and NF-κB were examined through the Western blotting. The Histopathological analysis of lung tissues of experimental animals was done. RESULTS The UV-vis spectroscopy and TEM examinations were proved the existence of CY-ZnONPs. The formulated CY-ZnONPs were displayed the potential antimicrobial activity. The supplementation of CY-ZnONPs were noticeably diminished the total protein and IL-6, IL-8, and TNF-α levels in the BALF of pneumonia mice. The ERK1/2, JNK1/2, and NF-κB expressions were appreciably diminished in the CY-ZnONPs supplemented mice. It also reduced the inflammatory cells penetration, and exhibited normal tissue arrangements in the lung tissues of pneumonia mice. CONCLUSION The findings of this investigation were proved that the synthesized CY-ZnONPs has the potential to ameliorate the M. pneumoniae infected pneumonia in investigational mice.

中文翻译：

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延胡索中的氧化锌纳米颗粒通过抑制MAPKs信号传导途径减轻了小鼠的支原体肺炎。

背景技术肺炎支原体（M.pneumoniae）占总肺炎发生率的3-10％。在最近的几十年中，金属纳米颗粒作为纳米抗生素被广泛研究。目的在这项研究中，我们打算检查（Corydalis yanhusuo）C. yanhusuo的氧化锌纳米颗粒（ZnONPs）对小鼠支原体感染的肺炎的治疗潜力。方法学ZnONPs是通过绿色途径技术制备的，并通过紫外可见光谱，透射电子显微镜，傅里叶变换红外技术和原子力显微镜进行了表征。通过良好的扩散法测试了配制的ZnONPs的抗菌活性。总蛋白白介素-1（IL-1），白介素-6（IL-6），白介素8（IL-8），采用试剂盒法检测肺炎支原体感染动物的BALF中肿瘤坏死因子α（TNF-α）和转化生长因子（TGF）的状态。通过Western印迹检测ERK1 / 2，JNK1 / 2和NF-κB的表达。进行了实验动物肺组织的组织病理学分析。结果紫外可见光谱和透射电镜检查证明了CY-ZnONPs的存在。所配制的CY-ZnONPs显示出潜在的抗菌活性。CY-ZnONPs的添加显着减少了肺炎小鼠BALF中的总蛋白和IL-6，IL-8和TNF-α水平。在补充CY-ZnONPs的小鼠中，ERK1 / 2，JNK1 / 2和NF-κB的表达明显降低。它还减少了炎症细胞的渗透，并且在肺炎小鼠的肺组织中表现出正常的组织排列。结论本研究的结果证明了合成的CY-ZnONP具有改善研究小鼠中肺炎支原体感染的肺炎的潜力。