Anthropogenic nutrient enrichment results in hypoxia, ocean acidification and elevated nutrients (HOAN) in coastal environments throughout the world. Here, we examined the composition of biofilm bacterial communities from a nutrient-excessive fish farm with low dissolved oxygen (DO) and pH levels using 16S rRNA gene sequencing. HOAN was accompanied by higher bacterial diversity and richness, and resulted in an altered community composition than the control site. HOAN resulted in more Flavobacteriales, Rhizobiales, Epsilonproteobacteria and Vibrionales, but less Oceanospirillales and Alteromonadales. Photobacterium sp. and Vibrio sp. were mostly found to be exclusive to HOAN conditions, suggesting that HOAN could possibly proliferate the presence of these potential pathogens. Our study suggests the complexity of bacterial communities to hypoxia and acidification in response to increased nutrient loads, along with identities of nutrient, oxygen and pH-susceptible bacterial groups that are most likely affected under this ocean trend.

人为的营养物富集会导致全世界沿海环境中的缺氧，海洋酸化和营养素升高（HOAN）。在这里，我们使用16S rRNA基因测序检查了营养过剩的养鱼场中低溶解氧（DO）和pH值低的生物膜细菌群落的组成。HOAN伴随着更高的细菌多样性和丰富性，并导致了社区组成比对照站点发生了变化。HOAN导致了更多的黄杆菌，根瘤菌，小肠杆菌和弧菌，但较少的海洋螺旋藻和交链藻。发光菌藻。和弧菌sp。大多数被发现是HOAN所独有的，这表明HOAN可能会扩散这些潜在病原体的存在。我们的研究表明，随着养分负荷的增加，细菌群落对缺氧和酸化的复杂性，以及在这种海洋趋势下最有可能受到影响的养分，氧气和pH敏感菌群的身份。