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Formation of functional innexin hemichannels, as well as gap junctional channels, in an insect cell line, NIAs-AeAl-2, derived from Asian tiger mosquito Aedes albopictus (Diptera: Culicidae): A partial but significant contribution of innexin 2.
Journal of Insect Physiology ( IF 2.3 ) Pub Date : 2020-05-21 , DOI: 10.1016/j.jinsphys.2020.104060
Ryoichi Yoshimura 1 , Taeko Suetsugu 2 , Ai Kawahara 1 , Kana Nakata 1 , Masato Shikata 1 , Souma Tanaka 1 , Tsutomu Ono 1 , Daisuke Fushiki 1 , Yasuhisa Endo 1
Affiliation  

In vertebrates, gap junctions and hemichannels consisting of connexins are important cell surface structures for communication with neighboring cells and for the regulation of various cell functions. To date, various gap-junction-related proteins have been found, including innexins in invertebrates and pannexins in vertebrates. Significant contributions of gap junctions by innexins and (hemi-)channels by pannexins to numerous functions have been reported. Verification of the presence and functional significance of innexin hemichannels, however, remains a gap in our knowledge in innexin physiology. In this study, we revealed the localization of an innexin protein (innexin 2) on the cell surface in mosquito tissues and cultured cells. Furthermore, we demonstrated the presence of functional hemichannels, as well as gap junctions, in mosquito cells using dye transfer assays. The inward uptake of fluorescent dye was inhibited by anti-innexin 2 antibody. These results suggest that innexin hemichannels are formed to function in cultured mosquito cells, in at least a partially innexin 2-dependent manner. Although only a few studies on insect hemichannels have been published, innexin-based hemichannels, as well as innexin gap junctions, could also significantly contribute to insect intercellular signal transduction.

中文翻译:

在源自亚洲虎蚊白纹伊蚊(Diptera:Culicidae)的昆虫细胞系NIAs-AeAl-2中形成功能性内毒素半通道以及间隙连接通道:内毒素2的部分但重要的贡献。

在脊椎动物中,由连接蛋白组成的间隙连接和半通道是重要的细胞表面结构,用于与邻近细胞通讯并调节各种细胞功能。迄今为止,已经发现了多种与间隙连接有关的蛋白质,包括无脊椎动物中的内毒素和脊椎动物中的pannexin。据报道,神经内毒素的间隙连接和泛尼毒素的(半)通道对许多功能的重要贡献。验证内源性半通道的存在和功能意义,仍然是我们对内源性生理学知识的一个空白。在这项研究中,我们揭示了一种内毒素蛋白(内毒素2)在蚊子组织和培养细胞的细胞表面上的定位。此外,我们展示了功能性半通道以及间隙连接的存在,使用染料转移试验检测蚊子细胞 抗内毒素2抗体抑制了荧光染料的内向吸收。这些结果表明,内毒素半通道以至少部分依赖内毒素2的方式在培养的蚊子细胞中起作用。尽管仅发表了很少的关于昆虫半通道的研究,但是基于内毒素的半通道以及内毒素间隙连接也可以显着促进昆虫细胞间信号转导。
更新日期:2020-05-21
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