Domains of unknown function protein family 1517 (DUF1517) in Ammopiptanthus mongolicus, could be induced by abiotic stresses, whose upstream regulatory sequence might be an ideal source of abiotic-induced promoter. In this study, a 1026-bp promoter of AmDUF1517 from A. mongolicus was cloned. Five deletion fragments (Full, Q1-Q4) of different length of the AmDUF1517 promoter were fused with the β-glucuronidase (GUS) reporter and transformed into Arabidopsis thaliana. The deletion analysis showed that sequences Full, Q1 and Q3 responded well to mannitol, NaCl and 4 °C stresses, while Q2 and Q4 segments did not. The Q3 fragment (280 bp; −280 to −1 bp) showed the highest promoter activity under normal and mannitol, NaCl and 4 °C conditions. The result suggested that Q3 in the AmDUF1517 gene promoter could be a new source of induced promoters for abiotic resistance breeding in plant genetic engineering.

未知功能蛋白家族1517（的结构域DUF1517中）沙冬青，可通过非生物胁迫，其上游调控序列可以是非生物诱导的启动子的理想来源来诱导。在这项研究中，克隆了来自蒙古曲霉的AmDUF1517的1026 bp启动子。将5个不同长度的AmDUF1517启动子缺失片段（Full，Q1-Q4）与β-葡糖醛酸糖苷酶（GUS）报告基因融合并转化为拟南芥。缺失分析表明，Full，Q1和Q3序列对甘露醇，NaCl和4°C胁迫反应良好，而Q2和Q4片段则没有。Q3片段（280 bp； -280至-1 bp）在正常和甘露醇，NaCl和4°C条件下显示出最高的启动子活性。结果表明，AmDUF1517基因启动子中的Q3可能是植物基因工程中非生物抗性育种的诱导启动子的新来源。