Gliomas account for 75% of the primary malignant brain tumors and a majority of lower-grade gliomas (LGG) inevitably develop into glioblastoma. The dysregulation of lncRNAs play a crucial role in LGG. In the present study, we first screened out six differentially expressed lncRNAs (AC021739.2, AL031722.1, AL354740.1, FGD5-AS1, LINC00844, and NEAT1) based on TCGA and GTEx RNA-seq databases. LncRNA prognostic signature was then established by Kaplan-Meier and multivariate Cox proportional hazards regression, with its predictive value validated by time-dependent receiver operating characteristic (ROC) curves. After lncRNA-miRNA-mRNA regulatory networks were established by Cytoscape 3.7.2, Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed, with results enriched in various malignancy-related functions and pathways. Finally, six putative drugs (irinotecan, camptothecin, mitoxantrone, azacitidine, mestranol, and enilconazole) were predicted by Connectivity Map. In conclusion, we identified a 6-lncRNA prognostic signature with its ceRNA networks, and six candidate drugs against LGG.

中文翻译：

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低级别胶质瘤中 ceRNA 网络和候选药物的六 lncRNA 预后特征的鉴定和验证。

胶质瘤占原发性恶性脑肿瘤的 75%，大多数低级别胶质瘤 (LGG) 不可避免地发展为胶质母细胞瘤。lncRNAs的失调在LGG中起着至关重要的作用。在本研究中，我们首先基于 TCGA 和 GTEx RNA-seq 数据库筛选出六种差异表达的 lncRNA（AC021739.2、AL031722.1、AL354740.1、FGD5-AS1、LINC00844 和 NEAT1）。然后通过 Kaplan-Meier 和多变量 Cox 比例风险回归建立 LncRNA 预后特征，其预测值通过时间相关的受试者工作特征 (ROC) 曲线验证。Cytoscape 3.7.2建立lncRNA-miRNA-mRNA调控网络后，进行基因肿瘤学（GO）和京都基因与基因组百科全书（KEGG）分析，结果丰富了各种与恶性肿瘤相关的功能和途径。最后，通过 Connectivity Map 预测了六种推定的药物（伊立替康、喜树碱、米托蒽醌、阿扎胞苷、美雌醇和恩康唑）。总之，我们通过其 ceRNA 网络确定了 6-lncRNA 预后特征，以及六种针对 LGG 的候选药物。