Abstract Gamma-amino butyric acid (GABA) has antihypertensive and anti-stress effects on human health. High hydrostatic pressure (HHP), a nonthermal treatment, has been documented as a novel approach to enhance the nutritional quality and functional components of plant-based food. In this study, a novel method to convert monosodium glutamate (MSG) to GABA using bee pollen as a natural catalyst was observed, and the GABA yield was improved by HHP treatment. Firstly, GABA production was confirmed by high performance liquid chromatography (HPLC) and tandem mass spectrometry (MS-MS), the highest GABA production was found in lotus bee pollen (LBP) when different varieties of bee pollen were used for the reaction. Secondly, the reaction conditions were utilized, and the optimal reaction condition of the GABA production was determined as described below: 250 mg/mL of bee pollen mixed with 590 mmol/L of MSG that prepared by 80 mmol/L of phosphate buffer (pH 5.8), and incubated at 40 °C for 6 h. For HHP treatment conditions, a treatment of 200 MPa for 10 min at 40 °C was found as the optimal. Under optimal conditions, GABA production reached 508.79 mmol/L (containing GABA from LBP) from 590 mmol/L of MSG. In conclusion, we found and optimized a novel method to convert monosodium glutamate (MSG) to GABA, and proposed the promotion of HHP on GABA bio-conversion.

中文翻译：

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使用蜂花粉作为新型环保催化剂，高静水压力增加了 γ-氨基丁酸的生物合成

摘要 γ-氨基丁酸（GABA）对人体健康具有抗高血压和抗应激作用。高静水压 (HHP) 是一种非热处理方法，已被证明是一种提高植物性食品营养质量和功能成分的新方法。在这项研究中，观察到了一种使用蜂花粉作为天然催化剂将味精 (MSG) 转化为 GABA 的新方法，并且通过 HHP 处理提高了 GABA 产量。首先，通过高效液相色谱 (HPLC) 和串联质谱 (MS-MS) 确认了 GABA 的产生，当使用不同品种的蜂花粉进行反应时，荷花蜂花粉 (LBP) 中的 GABA 产量最高。其次，利用反应条件，确定GABA生产的最佳反应条件如下：250 mg/mL 蜂花粉与 590 mmol/L 味精混合，由 80 mmol/L 磷酸盐缓冲液（pH 5.8）制备，并在 40 °C 下孵育 6 小时。对于 HHP 处理条件，发现在 40°C 下以 200 MPa 处理 10 分钟是最佳的。在最佳条件下，590 mmol/L MSG 的 GABA 产量达到 508.79 mmol/L（含 LBP 中的 GABA）。总之，我们发现并优化了一种将味精 (MSG) 转化为 GABA 的新方法，并提出了 HHP 对 GABA 生物转化的促进作用。79 mmol/L（含有来自 LBP 的 GABA）来自 590 mmol/L 的味精。总之，我们发现并优化了一种将味精 (MSG) 转化为 GABA 的新方法，并提出了 HHP 对 GABA 生物转化的促进作用。79 mmol/L（含有来自 LBP 的 GABA）来自 590 mmol/L 的味精。总之，我们发现并优化了一种将味精 (MSG) 转化为 GABA 的新方法，并提出了 HHP 对 GABA 生物转化的促进作用。