Eight diketopiperazines including five previously unreported derivatives were isolated from an endophytic fungus cultured from the medicinal plant Globularia alypum collected in Algeria. The strain was characterised by means of morphological studies and molecular phylogenetic methods and was found to represent a species of a new genus in the Chaetomiaceae, for which we propose the name Batnamyces globulariicola. The taxonomic position of the new genus, which appears phylogenetically related to Stolonocarpus and Madurella, was evaluated by a multi-locus genealogy and by morphological studies in comparison to DNA sequence data reported in the recent monographs of the family. The culture remained sterile on several culture media despite repeated attempts to induce sporulation, and only some chlamydospores were formed. After fermentation in submerged culture and extraction of the cultures with organic solvents, the major secondary metabolites of B. globulariicola were isolated and their chemical structures were elucidated by extensive spectral analysis including nuclear magnetic resonance (NMR) spectroscopy, high-resolution electrospray ionisation mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) measurements. The isolated compounds were tested for their biological activities against various bacteria, fungi, and two mammalian cell lines, but only three of them exhibited weak cytotoxicity against KB3.1 cells, but no antimicrobial effects were observed.

中文翻译：

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来自巴特木霉（Batnamyces globulariicola）的二酮哌拉嗪，gen。＆sp。十一月 （Chaetomiaceae），一种与阿尔及利亚药用植物Globularia alypum的根相关的真菌

从阿尔及利亚收集的药用植物球叶紫草培养的内生真菌中分离出八种二酮哌嗪，包括五种先前未报道的衍生物。该菌株通过形态学研究和分子系统发育方法进行了表征，并被发现为Chaetomiaceae的一个新属，我们将其命名为Batnamyces globulariicola。新属的分类学位置，在系统发生上与石竹和马杜氏菌有关通过多位点家谱和形态学研究，与该家族最近的专着中报道的DNA序列数据进行比较，评估了该基因。尽管反复尝试诱导孢子形成，但培养物在几种培养基上仍保持无菌状态，仅形成了部分衣原体孢子。在深层培养中发酵并用有机溶剂提取培养物后，球孢双歧杆菌的主要次生代谢产物分离并通过广泛的光谱分析，包括核磁共振（NMR）光谱，高分辨率电喷雾电离质谱（HRESIMS）和电子圆二色性（ECD）测量，阐明了它们的化学结构。测试了分离出的化合物对各种细菌，真菌和两种哺乳动物细胞系的生物学活性，但只有其中三种对KB3.1细胞表现出较弱的细胞毒性，但未观察到抗菌作用。