Ecotoxicological bioassays have been widely utilized to evaluate the toxicity of substances to organisms. However, the main challenge for researchers is finding native species to assess the effects of pollutants on aquatic biota. The tropical Oligochaeta, Allonais inaequalis, can be used as a test organism in bioassays to understand the effects of toxicants on aquatic ecosystems and their impact on native aquatic biota. In this study, we tested four methodological designs to validate the use of our "Allonais inaequalis reproduction test" as an ecotoxicological bioassay. For each sample, the assay consisted of a bottle containing 10 mg of sterilized fine sand, 60 mL of dechlorinated tap water and 6 organisms, fed at the beginning of the test and again after 5 days. The assay was first established in a controlled environment and then used to evaluate a stressed environment containing one of the following three toxicants suggested by the OECD (2008) and Corbi et al. (2015): zinc chloride, copper sulfate, or potassium chloride. Our results showed that the best experimental design for reproduction analysis was a static, long-term bioassay, which lasted 10 days without aeration and allowed for the reproduction of multiple generations (10 ± 5 new organisms). The observed inhibition reproduction by toxicants (EC50 ranging between 0.2 mg L-1 and 1.36 g L-1) validated the methods used in this paper. The use of a reproduction endpoint is a new contribution to the ecotoxicological toolbox, examining responses from a native organism to predict the effects of pollutants in an aquatic environment.

中文翻译：

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阿洛奈斯不等生殖繁殖试验作为生态毒理生物测定的用途。

生态毒理生物测定法已被广泛用于评估物质对生物体的毒性。但是，研究人员面临的主要挑战是寻找原生物种来评估污染物对水生生物的影响。热带的Oligochaeta，Allonais inaequalis，可以用作生物测定中的测试生物，以了解有毒物质对水生生态系统的影响及其对天然水生生物群的影响。在这项研究中，我们测试了四种方法设计，以验证我们的“异位阿洛奈斯繁殖试验”作为一种生态毒理学生物测定法的使用。对于每个样品，测定均由一个装有10 mg无菌细砂，60 mL脱氯自来水和6种生物的瓶子组成，在试验开始时和5天后再次饲喂。该方法首先在可控环境中建立，然后用于评估包含OECD（2008）和Corbi等人建议的以下三种有毒物质之一的压力环境。（2015）：氯化锌，硫酸铜或氯化钾。我们的结果表明，用于繁殖分析的最佳实验设计是静态的长期生物测定法，该实验可连续进行10天而不充气，并且可以进行多代繁殖（10±5个新生物）。观察到的有毒物质的抑制繁殖（EC50在0.2 mg L-1和1.36 g L-1之间）验证了本文所使用的方法。繁殖终点的使用是对生态毒理学工具箱的新贡献，它可以检查原生生物的反应来预测水生环境中污染物的影响。