The oviduct is a dynamic organ that suffers changes during the oestrous cycle and modulates gamete and embryo physiology. We analyse the possible existence of Protein kinase A (PKA)-dependent hormone-regulated pathways in porcine ampulla and primary cell cultures by 2D-electrophoresis/Western blot using anti-phospho PKA substrate antibodies. Differential phosphorylation was observed for ten proteins that were identified by mass spectrometry. The results were validated for five of the proteins: Annexin A5, Calumenin, Glyoxalase I and II and Enolase I. Immunofluorescence analyses show that Calumenin, Glyoxalase II and Enolase I change their localisation in the oviductal epithelium through the oestrus cycle. The results demonstrate the existence of PKA hormone-regulated pathways in the ampulla epithelium during the oestrus cycle.

中文翻译：

---

猪输卵管上皮细胞中激素调节的PKA活性

输卵管是一个动态器官，在发情周期中会发生变化并调节配子和胚胎生理。我们使用抗磷酸 PKA 底物抗体通过二维电泳/蛋白质印迹分析了猪壶腹和原代细胞培养物中蛋白激酶 A (PKA) 依赖性激素调节途径的可能存在。对通过质谱法鉴定的十种蛋白质观察到差异磷酸化。对五种蛋白质的结果进行了验证：膜联蛋白 A5、Calumen、Glyoxalase I 和 II 以及 Enolase I。免疫荧光分析表明 Calumenin、Glyoxalase II 和 Enolase I 通过发情周期改变它们在输卵管上皮中的定位。结果表明在发情周期期间壶腹上皮中存在 PKA 激素调节途径。