The objective of this study is to discern the proteomic differences responsible for hampering the receptivity of endometrium and subduing the fertility of females with polycystic ovary syndrome in analogy to healthy fertile females. This study was designed in collaboration with Hakeem Abdul Hameed Centenary Hospital affiliated to Jamia Hamdard, New Delhi, India. Serum samples were taken from infertile PCOS subjects ( n = 6) and fertile control subjects ( n = 6) whereas endometrial tissue samples were recruited from ovulatory PCOS ( n = 4), anovulatory PCOS ( n = 4) and normal healthy fertile control subjects ( n = 4) for proteomic studies. Additionally, endometrial biopsies from healthy fertile control ( n = 8), PCOS with infertility ( n = 6), unexplained infertility ( n = 3) and endometrial hyperplasia ( n = 3) were taken for validation studies. Anthropometric, biochemical and hormonal evaluation was done for all the subjects enrolled in this study. Protein profiles were generated through 2D-PAGE and differential proteins analyzed with PD-QUEST software followed by identification with MALDI-TOF MS protein mass fingerprinting. Validation of identified proteins was done through RT-PCR relative expression analysis. Protein profiling of serum revealed differential expression of proteins involved in transcriptional regulation, embryogenesis, DNA repair, decidual cell ploidy, immunomodulation, intracellular trafficking and degradation processes. Proteins involved in cell cycle regulation, cellular transport and signaling, DNA repair, apoptotic processes and mitochondrial metabolism were found to be differentially expressed in endometrium. The findings of this study revealed proteins that hold strong candidature as potential drug targets to regulate the cellular processes implicating infertility and reduced receptivity of endometrium in women with polycystic ovary syndrome.

中文翻译：

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通过血清和子宫内膜轮廓的蛋白质组学筛选揭示了与多囊卵巢综合征女性生育能力减弱和子宫内膜容受性降低相关的特征蛋白

本研究的目的是辨别导致子宫内膜容受性和抑制多囊卵巢综合征女性生育能力的蛋白质组学差异，类似于健康的生育女性。本研究是与印度新德里 Jamia Hamdard 附属的 Hakeem Abdul Hameed Centenary Hospital 合作设计的。血清样本取自不育 PCOS 受试者 (n = 6) 和可育对照受试者 (n = 6)，而子宫内膜组织样本来自排卵 PCOS (n = 4)、无排卵 PCOS (n = 4) 和正常健康可育对照受试者( n = 4) 用于蛋白质组学研究。此外，还对健康生育对照 (n = 8)、PCOS 不孕 (n = 6)、不明原因不孕 (n = 3) 和子宫内膜增生 (n = 3) 的子宫内膜活检进行了验证研究。对参与本研究的所有受试者进行了人体测量学、生化和激素评估。通过 2D-PAGE 生成蛋白质谱，并使用 PD-QUEST 软件分析差异蛋白质，然后使用 MALDI-TOF MS 蛋白质质量指纹识别进行鉴定。通过 RT-PCR 相对表达分析对鉴定的蛋白质进行验证。血清的蛋白质分析揭示了参与转录调控、胚胎发生、DNA 修复、蜕膜细胞倍性、免疫调节、细胞内运输和降解过程的蛋白质的差异表达。发现参与细胞周期调节、细胞转运和信号传导、DNA 修复、凋亡过程和线粒体代谢的蛋白质在子宫内膜中差异表达。