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An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics.
Biomedical Microdevices ( IF 3.0 ) Pub Date : 2020-05-21 , DOI: 10.1007/s10544-020-00494-4
Wenbo Yang 1 , Dianlong Yang 1 , Shisong Gong 1 , Xiaobing Dong 1 , Luyao Liu 1 , Shengda Yu 1 , Xiaolei Zhang 2 , Shengxiang Ge 3 , Dong Wang 2 , Ningshao Xia 3 , Duli Yu 1, 4 , Xianbo Qiu 1
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Currently, most HIV tests are performed with blood samples, or alternatively saliva samples are used for HIV testing. Simple HIV tests need to be performed in hospitals or other medical agencies instead of more invasive HIV blood tests. To enable point-of-care (POC) HIV diagnostics, based on a recently developed lateral flow strip for HIV urine testing, a microfluidic immunoassay cassette with a handheld optical reader is developed. Based on lateral flow strip with gold colloid reporter, the integrated immunoassay cassette can perform sample introduction, metering, discharging, applying and detection which simplifies HIV testing. An indicator is incorporated into the cassette to guide sample introduction based on color change, and further, the excess test sample is stored inside the sealed cassette to avoid any contamination. The low-cost handheld optical reader can provide a test result within a few seconds, which is useful for simple, sensitive and affordable HIV onsite detection. Instead of using normal white LEDs, a customized back light module embedded with green LEDs is adopted to illuminate the lateral flow strip with an appropriate working current to achieve optimal performance. Compared to the standard lateral flow strips using a benchtop reader, with the disposable immunoassay cassette assisted by the handheld optical reader, more convenient, easier-to-operate, and more affordable HIV urine testing can be achieved in POC diagnostics.

中文翻译:

带有手持读取器的免疫分析盒,用于即时诊断中的HIV尿液测试。

当前,大多数艾滋病毒检测都是用血液样本进行的,或者唾液样本也用于艾滋病毒检测。需要在医院或其他医疗机构中执行简单的HIV测试,而不是更具侵入性的HIV血液测试。为了实现即时护理(POC)HIV诊断,基于最近开发的用于HIV尿液检测的侧向流动条,开发了带有手持式光学读取器的微流免疫测定盒。集成的免疫测定盒基于带有金胶报告物的侧流试纸条,可以进行样品引入,计量,排放,施加和检测,从而简化了HIV检测。指示器结合在盒中以根据颜色变化引导样品导入,此外,多余的测试样品存储在密封盒中以避免任何污染。低成本手持式光学阅读器可以在几秒钟内提供测试结果,这对于简单,灵敏且价格合理的HIV现场检测很有用。代替使用普通的白色LED,采用了嵌入有绿色LED的定制背光模块,以适当的工作电流照亮侧流条,以实现最佳性能。与使用台式读取器的标准侧向流动条相比,借助手持式光学读取器辅助的一次性免疫测定盒,可以在POC诊断中实现更方便,更易操作且更实惠的HIV尿液检测。采用内置绿色LED的定制背光模块,以适当的工作电流照亮侧流条,以实现最佳性能。与使用台式读取器的标准侧向流动条相比,借助手持式光学读取器辅助的一次性免疫测定盒,可以在POC诊断中实现更方便,更易于操作且更实惠的HIV尿液检测。采用内置绿色LED的定制背光模块,以适当的工作电流照亮侧流条,以实现最佳性能。与使用台式读取器的标准侧向流动条相比,借助手持式光学读取器辅助的一次性免疫测定盒,可以在POC诊断中实现更方便,更易于操作且更实惠的HIV尿液检测。
更新日期:2020-05-21
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