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Color Fourier ptychographic microscopy based on symmetrical illumination and wavelength multiplexing
Journal of Optics ( IF 2.0 ) Pub Date : 2020-05-19 , DOI: 10.1088/2040-8986/ab8c12
Muyang Zhang , Di Yang , Yanmei Liang

Fourier ptychographic microscopy (FPM), by using a LED array as its light source and each LED being lit up sequentially, can recover a high resolution (HR) and large field of view image from a sequence of low resolution (LR) images. It is difficult to obtain a perfect high resolution color image by a monochrome camera because of non-overlapping focal planes of different color channels resulting from the residual chromatic aberration of the microscopic objective. In this paper, a kind of method, based on symmetrical LED illumination and wavelength multiplexing, is applied in a FPM system to collect LR images and recover HR color images. Compared with single LED illumination, symmetrical illumination has higher defocus tolerance. It is proven by simulated and experimental results that higher-quality HR color images can be obtained based on wavelength multiplexing and symmetrical illumination by a monochrome camera when defocus or chromatic aberration exist. It is demonstrated that...

中文翻译:

基于对称照明和波长多路复用的彩色傅里叶指纹图谱显微镜

通过使用LED阵列作为光源并且每个LED依次点亮,傅里叶液相色谱(FPM)可以从一系列低分辨率(LR)图像中恢复高分辨率(HR)和大视野图像。由于单色物镜的残余色差导致的不同颜色通道的不重叠焦平面,使得单色相机难以获得完美的高分辨率彩色图像。本文将一种基于对称LED照明和波长复用的方法应用于FPM系统中,以采集LR图像并恢复HR彩色图像。与单一LED照明相比,对称照明具有更高的散焦容限。通过仿真和实验结果证明,当存在散焦或色差时,基于单色相机的波长复用和对称照明,可以获得更高质量的HR彩色图像。证明...
更新日期:2020-05-19
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