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Boronic acid-engineered gold nanoparticles for cytosolic protein delivery.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-05-20 , DOI: 10.1039/d0bm00679c
Song Zhang 1 , Yiyun Cheng
Affiliation  

Cytosolic protein delivery technique plays an important role in protein-based biotechnologies and therapeutics. However, the development of efficient nanocarriers for delivering cargo proteins into cytosols remains a continuing challenge due to the existence of multiple barriers. Here, we report an efficient strategy for the cytosolic delivery of native proteins by surface-engineered gold nanoparticles combined with hypertonicity treatment. Sub-10 nm gold nanoparticles stabilized by both cysteamine and 4-mercaptophenylboronic acid were used to complex cargo proteins via a combination of nitrogen-boronate coordination and ionic interactions. The yielding protein complexes with a size around 100 nm showed efficient endocytosis via micropinocytosis- and lipid raft-mediated pathways. Further the hypertonicity treatment of the transduced cells by glycerol, glucose, sucrose, and NaCl solutions efficiently facilitates the endosomal escape and the intracellular release of cargo proteins. By the proposed strategy, cargo proteins including bovine serum albumin, ovalbumin, green fluorescent protein, R-phycoerythrin, and horseradish peroxidase were successfully delivered into cell cytosol with maintained protein bioactivity. This study provides a feasible and efficient strategy for the intracellular protein delivery.

中文翻译:

硼酸工程金纳米颗粒用于胞质蛋白的递送。

胞质蛋白递送技术在基于蛋白质的生物技术和治疗剂中起着重要作用。然而,由于存在多种屏障,用于将货物蛋白递送到胞质溶胶中的有效纳米载体的开发仍然是持续的挑战。在这里,我们报告通过表面工程金纳米粒子结合高渗治疗的天然蛋白的胞质传递的有效策略。通过半胱胺和4-巯基苯基硼酸稳定的亚10纳米金纳米颗粒通过氮-硼酸酯配位和离子相互作用的组合用于复杂的货物蛋白。产生的蛋白质复合物的大小约为100 nm,通过微胞饮作用和脂筏介导的途径。进一步地,用甘油,葡萄糖,蔗糖和NaCl溶液对转导细胞的高渗处理有效地促进了内体逃逸和货物蛋白的细胞内释放。通过提出的策略,将包括牛血清白蛋白,卵清蛋白,绿色荧光蛋白,R-藻红蛋白和辣根过氧化物酶在内的货物蛋白成功地传递到细胞溶胶中,并保持了蛋白质的生物活性。这项研究为细胞内蛋白传递提供了一种可行而有效的策略。
更新日期:2020-06-30
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