A Near-Infrared Fluorescent Probe Coated with β-Cyclodextrin Molecules for Real-Time Imaging-Guided Intraoperative Ureteral Identification and Diagnosis.,Molecular Pharmaceutics

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A Near-Infrared Fluorescent Probe Coated with β-Cyclodextrin Molecules for Real-Time Imaging-Guided Intraoperative Ureteral Identification and Diagnosis.
Molecular Pharmaceutics ( IF 4.5 ) Pub Date : 2020-05-19 , DOI: 10.1021/acs.molpharmaceut.0c00364
Katsunori Teranishi ₁

Affiliation

Although iatrogenic ureteral injury and its lack of recognition due to ureteral invisibility are serious incidents in open and laparoscopic abdominal surgeries, there are currently no safe and effective methods for intraoperative ureteral identification (IUI) and diagnosis (IUD). In this study, I designed and chemically synthesized a near-infrared fluorescence (NIRF) imaging probe (CD-NIR-1) and evaluated its clearance and ability for IUI and IUD in animal models. CD-NIR-1 demonstrated high specificity and ultrarapid clearance by rat kidneys to the urinary bladder following intravenous administration of a single dose (25 nmol/kg of body weight), with 96% of the dose ultimately excreted at the first urination with no chemical modification. Furthermore, urine containing CD-NIR-1 in ureters showed strong NIRF, thereby enabling IUI and IUD via NIRF imaging. These results demonstrated the efficacy of CD-NIR-1 for clinical use.

中文翻译：

涂有β-环糊精分子的近红外荧光探针，用于实时成像引导的术中输尿管识别和诊断。

尽管在开放式和腹腔镜腹部手术中，医源性输尿管损伤及其因输尿管不可见而引起的识别不足是严重的事件，但目前尚无安全有效的术中输尿管识别（IUI）和诊断（IUD）方法。在这项研究中，我设计并化学合成了近红外荧光（NIRF）成像探针（CD-NIR-1），并评估了其在动物模型中的清除率以及IUI和IUD的能力。CD-NIR-1具有静脉注射单剂量（25 nmol / kg体重）后大鼠肾脏对膀胱的高特异性和超快速清除率，其中96％的剂量在第一次排尿时最终排泄而没有化学药品修改。此外，输尿管中含有CD-NIR-1的尿液表现出较强的NIRF，从而通过NIRF成像启用IUI和IUD。这些结果证明了CD-NIR-1在临床上的功效。

更新日期：2020-07-06

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