Biosensor devices are important in clinical practice and environmental studies because they allow specific detection of target molecules from a variety of samples. However, challenges still remain when attempting to develop sensitive, selective, rapid, and cost-effective assays for biomolecule detection. Devices that use DNA-modifying enzymes to catalyze detection reactions have recently been developed. These devices show promise because they are often more sensitive and specific, have shorter assay times, are more cost-effective, and are easier to use than the currently used biosensors. Here, we review the current trends in DNA-modifying enzyme reaction-coupled biosensors, including devices using DNA polymerases, nicking endonuclease, exonucleases, and ligases. The molecular strategies underlying diverse DNA-modifying enzymes coupled to biomolecule detection platforms are reviewed. We also discuss the strengths and limitations of each strategy and suggest methods to overcome current limitations. Finally, the future prospects of DNA-modifying enzyme reaction-coupled biosensor development have been proposed.

中文翻译：

---

用于疾病诊断的基于DNA修饰酶反应的生物传感器：最近的生物技术进展和未来展望。

生物传感器设备在临床实践和环境研究中非常重要，因为它们可以从各种样品中特异性检测目标分子。但是，在尝试开发灵敏，选择性，快速且经济高效的生物分子检测方法时，挑战仍然存在。最近已经开发出使用DNA修饰酶催化检测反应的设备。这些设备显示出了希望，因为它们通常比目前使用的生物传感器更灵敏，更特异性，化验时间更短，更具成本效益并且更易于使用。在这里，我们回顾了DNA修饰酶反应耦合生物传感器的当前趋势，包括使用DNA聚合酶，切口内切核酸酶，核酸外切酶和连接酶的设备。综述了与生物分子检测平台偶联的多种DNA修饰酶的分子策略。我们还将讨论每种策略的优势和局限性，并提出克服当前局限性的方法。最后，提出了DNA修饰酶反应耦合生物传感器的发展前景。