In recent years, the number of new antimicrobial drugs launched on the market has decreased considerably even though there has been an increase in the number of resistant microbial strains. Thus, antimicrobial resistance has become a serious public health problem. Amphibian skin secretions are a rich source of host defense peptides, which generally are cationic and hydrophobic molecules, with a broad-spectrum of activity. In this study, one novel multifunctional defense peptide was isolated from the skin secretion of the Chaco tree frog, Boana raniceps. Figainin 2 (1FLGAILKIGHALAKTVLPMVTNAFKPKQ28) is cationic and hydrophobic, adopts an α-helical structure in 50% (v/v) trifluoroethanol (TFE), and is thermally stable. This peptide exhibited activity against Gram-negative and Gram-positive pathogenic bacteria arboviruses, T. cruzi epimastigotes; however, it did not show activity against yeasts. Figainin 2 also showed antiproliferative activity on cancer cells, is moderately active on human erythrocytes, and activates the oxidative burst in human neutrophils.

中文翻译：

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查科树蛙 Boana raniceps 皮肤分泌物中新型多功能宿主防御肽的生物学特性。


近年来，尽管耐药菌株数量有所增加，但上市的新抗菌药物数量却大幅减少。因此，抗菌药物耐药性已成为严重的公共卫生问题。两栖动物皮肤分泌物是宿主防御肽的丰富来源，它们通常是阳离子和疏水性分子，具有广谱活性。在这项研究中，从查科树蛙 Boana raniceps 的皮肤分泌物中分离出一种新型多功能防御肽。 Figainin 2 (1FLGAILKIGHALAKTVLPMVTNAFKPKQ28) 是阳离子和疏水性的，在 50% (v/v) 三氟乙醇 (TFE) 中采用 α-螺旋结构，并且具有热稳定性。该肽对革兰氏阴性和革兰氏阳性病原菌虫媒病毒、克氏锥虫上鞭毛体具有活性；然而，它没有表现出针对酵母的活性。 Figainin 2 还表现出对癌细胞的抗增殖活性，对人红细胞具有中等活性，并激活人中性粒细胞的氧化爆发。