Long non‐coding RNAs (lncRNAs) are involved in the resistance of plants to infection by pathogens via interactions with microRNAs (miRNAs). Long non‐coding RNAs are cleaved by miRNAs to produce phased small interfering RNAs (phasiRNAs), which, as competing endogenous RNAs (ceRNAs), function as decoys for mature miRNAs, thus inhibiting their expression, and contain pre‐miRNA sequences to produce mature miRNAs. However, whether lncRNAs and miRNAs mediate other molecular mechanisms during plant resistance to pathogens is unknown. In this study, as a positive regulator, Sl‐lncRNA15492 from tomato (Solanum lycopersicum Zaofen No. 2) plants affected tomato resistance to Phytophthora infestans. Gain‐ and loss‐of‐function experiments and RNA ligase‐mediated 5′‐amplification of cDNA ends (RLM‐5′ RACE) also revealed that Sl‐miR482a was negatively involved in tomato resistance by targeting Sl‐NBS‐LRR genes and that silencing of Sl‐NBS‐LRR1 decreased tomato resistance. Sl‐lncRNA15492 inhibited the expression of mature Sl‐miR482a, whose precursor was located within the antisense sequence of Sl‐lncRNA15492. Further degradome analysis and additional RLM‐5′ RACE experiments verified that mature Sl‐miR482a could also cleave Sl‐lncRNA15492. These results provide a mechanism by which lncRNAs might inhibit precursor miRNA expression through antisense strands of lncRNAs, and demonstrate that Sl‐lncRNA15492 and Sl‐miR482a mutually inhibit the maintenance of Sl‐NBS‐LRR1 homeostasis during tomato resistance to P. infestans.

中文翻译：

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S1-lncRNA15492与S1-miR482a相互作用并影响茄属茄科抗疫霉菌的免疫力。

长非编码RNA（lncRNA）通过与microRNA（miRNA）的相互作用参与植物对病原体感染的抗性。长的非编码RNA被miRNA切割，产生分阶段的小干扰RNA（phasiRNA），后者作为竞争性内源RNA（ceRNA），充当成熟miRNA的诱饵，从而抑制了它们的表达，并包含pre-miRNA序列以产生成熟的miRNA。 miRNA。但是，尚不清楚lncRNA和miRNA在植物对病原体的抗性过程中是否介导其他分子机制。在这项研究中，作为阳性调节剂，来自番茄（茄子Zaofen 2号）的Sl-lncRNA15492影响了番茄对疫霉的抗性。功能获得和丧失功能实验以及RNA连接酶介导的cDNA末端5'扩增（RLM-5'RACE）还显示，Sl-miR482a通过靶向Sl - NBS-LRR基因而负参与番茄抗性，并且Sl ‐ NBS‐LRR1沉默降低番茄抗性。SllncRNA15492抑制成熟的SlmiR482a的表达，后者的前体位于SllncRNA15492的反义序列内。进一步的降解组分析和其他RLM-5'RACE实验证明，成熟的Sl-miR482a也可以切割Sl-lncRNA15492。这些结果提供了lncRNA可能通过lncRNA的反义链抑制前体miRNA表达的机制，并证明了Sl-lncRNA15492和Sl-miR482a相互抑制番茄对致病疫霉的抵抗过程中Sl-NBS-LRR1稳态的维持。