Dammarane sapogenins (DS), an extract derived from ginseng by alkaline hydrolysis of total ginsenosides, possesses high pharmacological activity and higher bioavailability than ginsenosides. The present study was designed to investigate the anxiolytic‐like effects of DS in a mouse model of chronic social defeat stress (CSDS). DS (40 and 80 mg/kg) significantly ameliorated social avoidance and anxiety‐like behavior in four test models of CSDS, showing increased time in the interaction zone in the social interaction test and in the center of the field in the open field test, an increased percentage of entries and open arm time in the elevated plus maze, and reduced latency to eat in the novelty‐suppressed feeding test. Biochemical analyses showed that DS significantly reduced serum corticosterone levels and increased brain concentration of neurotransmitter 5‐HT and noradrenaline in CSDS mice. Treatment with DS significantly upregulated BDNF (brain‐derived neurotrophic factor), p‐CREB/CREB and p‐ERK1/2/ERK1/2 protein expression in the hippocampus and prefrontal cortex of CSDS mice. Collectively, these results suggest that DS exerts anxiolytic‐like effects in CSDS model mice and the action is mediated, at least in part, by modulating the HPA (hypothalamic–pituitary–adrenal) axis and monoamine neurotransmitter levels, and via ERK/CREB/BDNF signaling pathway.

中文翻译：

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Dammarane 皂苷元通过上调 ERK / CREB ​​/ BDNF 通路减轻压力引起的焦虑样行为

Dammarane sapogenins (DS) 是一种通过碱性水解人参总皂苷而从人参中提取的提取物，具有比人参皂苷更高的药理活性和更高的生物利用度。本研究旨在研究 DS 在慢性社交失败压力 (CSDS) 小鼠模型中的抗焦虑作用。DS（40 和 80 mg/kg）显着改善了 CSDS 的四种测试模型中的社交回避和焦虑样行为，显示在社交互动测试中的互动区和在旷场测试中在场中心的时间增加，在高架十字迷宫中增加了进入和开放臂时间的百分比，并在新奇抑制喂养测试中减少了进食的潜伏期。生化分析表明，DS 显着降低了 CSDS 小鼠的血清皮质酮水平，并增加了神经递质 5-HT 和去甲肾上腺素的脑浓度。DS 治疗显着上调 CSDS 小鼠海马和前额叶皮层中的 BDNF（脑源性神经营养因子）、p-CREB/CREB ​​和 p-ERK1/2/ERK1/2 蛋白表达。总的来说，这些结果表明 DS 在 CSDS 模型小鼠中发挥抗焦虑样作用，并且该作用至少部分是通过调节 HPA（下丘脑-垂体-肾上腺）轴和单胺类神经递质水平以及通过 ERK/CREB/ BDNF 信号通路。CSDS 小鼠海马和前额叶皮层中 p-CREB/CREB ​​和 p-ERK1/2/ERK1/2 蛋白的表达。总的来说，这些结果表明 DS 在 CSDS 模型小鼠中发挥抗焦虑样作用，并且该作用至少部分是通过调节 HPA（下丘脑-垂体-肾上腺）轴和单胺类神经递质水平以及通过 ERK/CREB/ BDNF 信号通路。CSDS 小鼠海马和前额叶皮层中 p-CREB/CREB ​​和 p-ERK1/2/ERK1/2 蛋白的表达。总的来说，这些结果表明 DS 在 CSDS 模型小鼠中发挥抗焦虑样作用，并且该作用至少部分是通过调节 HPA（下丘脑-垂体-肾上腺）轴和单胺类神经递质水平以及通过 ERK/CREB/ BDNF 信号通路。