Microalgae exhibit extensive potential for counteracting imminent challenges in the nutraceutical, pharmaceutical, and biomaterial sectors, but lack economic viability. Biotechnological systems for contamination control could advance the economic viability of microalgal feedstock, but the selection of suitable strains that sustainably promote microalgal productivity remains challenging. In this study, total diversity in phototrophic Chlorella vulgaris cultures was assessed by amplicon sequencing comparing cultures subjected to five different cultivation conditions. Overall, 12 eukaryotic and 53 prokaryotic taxa were identified; Alphaproteobacteria (36.7%) dominated the prokaryotic and C. vulgaris (97.2%) the eukaryotic community. Despite altering cultivation conditions, 2 eukaryotic and 40 prokaryotic taxa remained stably associated with C. vulgaris; diversity between systems did not significantly differ (P > 0.05). Among those, 20 cultivable taxa were isolated and identified by 16S rDNA sequencing. Subsequently, controlled co‐cultures were investigated showing stable associations of C. vulgaris with Sphingopyxis sp. and Pseudomonas sp.. Out‐competition of C. vulgaris due to ammonium or phosphate limitation was not observed, despite significantly elevated growth of Sphingopyxis sp. and Tistrella sp.. (P < 0.05). Nevertheless, C. vulgaris growth was impaired by Tistrella sp.. Hence, the study provides a selection of stable indigenous prokaryotes and eukaryotes for artificially tailoring microbial biocenoses. Following a bottom‐up approach, it provides a base for controlled co‐cultures and thus the establishment of even more complex biocenoses using interkingdom assemblages. Such assemblages can benefit from functional richness for improved nutrient utilization, as well as bacterial load control, which can enhance microalgal feedstock production through improved culture stability and productivity.

中文翻译：

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小球藻（Trebouxiophyceae）相关微生物群落的特征1。

微藻具有应对营养，制药和生物材料领域迫在眉睫的挑战的巨大潜力，但缺乏经济可行性。用于污染控制的生物技术系统可以提高微藻原料的经济可行性，但是如何选择能够持续提高微藻生产力的合适菌株仍然具有挑战性。在这项研究中，通过比较接受五种不同培养条件的培养物的扩增子测序，评估了光养性小球藻培养物的总多样性。总体而言，鉴定出了12个真核生物分类群和53个原核生物分类群。丙酸杆菌（36.7％）主导原核和寻常梭状芽胞杆菌（97.2％）真核生物社区。尽管栽培条件发生了变化，但仍有2个真核生物和40个原核生物分类单元与寻常梭状芽胞杆菌稳定相关。系统之间的多样性没有显着差异（P  > 0.05）。其中，通过16S rDNA测序分离并鉴定了20种可培养的类群。随后，对受控的共培养物进行了研究，结果显示寻常型梭状芽胞杆菌与Sphingopyxis sp。有稳定的联系。和假单胞菌属的.. OUT-竞争普通小球藻由于铵或磷酸盐限制未观察到，尽管显著升高生长鞘脂单胞菌属。和Tistrella sp ..（P <0.05）。尽管如此，小球藻生长通过受损Tistrella SP ..因此，研究提供了一种用于人工剪裁微生物biocenoses选择稳定土著原核生物和真核生物的。遵循自下而上的方法，它为受控的共培养提供了基础，因此可以通过相互融合来建立更复杂的生物谱图。此类组合可受益于功能丰富的营养元素，可提高营养利用率，并控制细菌负荷，可通过改善培养物的稳定性和生产率来提高微藻原料的生产。