Development of Miniaturized Electrochemiluminescence Instrument using Multi‐pixel Photon Counter as the Optical Detector,Electroanalysis

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Development of Miniaturized Electrochemiluminescence Instrument using Multi‐pixel Photon Counter as the Optical Detector
Electroanalysis ( IF 2.7 ) Pub Date : 2020-05-20 , DOI: 10.1002/elan.202000094
Wanlin Dai ₁ , Wenyuan Zhao ₁ , Ying Ma ₁ , Jianshan Ye ₁ , Jiye Jin ₂

Affiliation

We developed a miniaturized electrochemiluminescence (ECL) instrument coupled with a light‐emitting diode‐based bipolar electrochemical sensor (LED‐BPES). This instrument composes of a microcontroller circuit, a power supply circuit, a potentiostat, an optical detecting circuit, and a communication circuit. The multi‐pixel photon counter (MPPC), which is low‐cost, small‐size, and wide‐range in optical measurements, is chosen as the optical detector. The LED‐BPES composes of a disposable screen‐printed carbon electrode (SPCE) and a surface‐mount red LED. Depended on the closed bipolar electrode (C‐BPE) structure, the LED‐BPES not only avoids the employment of unstable and complex ECL reactions but also offers a cost‐effective alternative for the over‐priced ECL reagents by using a mini‐size commercial LED as the luminescent producer. The combination of MPPC and LED‐BPES helps to set up the simplified and downsized instrument system with low price and high efficiency. The presented instrument coupled with LED‐BPES works excellent in electroactive molecules detection and has great potential in the application of heavy metal ions detection.

中文翻译：

用多像素光子计数器作为光学探测器的小型化电化学发光仪的研制

我们开发了一种小型化的电化学发光（ECL）仪器，并结合了基于发光二极管的双极电化学传感器（LED-BPES）。该仪器由微控制器电路，电源电路，稳压器，光学检测电路和通信电路组成。选择光学测量的低成本，小尺寸和宽范围的多像素光子计数器（MPPC）作为光学检测器。LED-BPES由一次性丝网印刷碳电极（SPCE）和表面安装红色LED组成。根据封闭的双极电极（C-BPE）的结构，LED-BPES不仅避免了不稳定和复杂的ECL反应的发生，而且还通过使用微型商业产品为高价ECL试剂提供了一种经济高效的替代品LED作为发光体。MPPC和LED-BPES的结合有助于以低成本和高效率建立简化和小型化的仪器系统。提出的仪器与LED-BPES结合使用，在电活性分子检测方面表现出色，在重金属离子检测应用中具有巨大潜力。

更新日期：2020-05-20

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