Biosynthesis of bioactive tamarixetin in recombinant Escherichia coli,Biotechnology and Applied Biochemistry

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Biosynthesis of bioactive tamarixetin in recombinant Escherichia coli
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-05-19 , DOI: 10.1002/bab.1958
Sumangala Darsandhari ₁ , Dipesh Dhakal ₁ , Biplav Shrestha ₁ , Sanghun Lee ₁ , Narae Jung ₁ , Hye Jin Jung ₁ , Jae Kyung Sohng _{1,

2}

Affiliation

Tamarixetin, a monomethylated derivative of quercetin, has been reported to possess many important biological activities. In the present study, a whole cell biotransformation system was used for regiospecific methylation of quercetin to produce 4′-O-methylated quercetin (tamarixetin) using methyltransferase from Streptomyces sp. KCTC 0041BP in Escherichia coli Bl21 (DE3). Its production was enhanced by adding a plasmid containing S-adenosine-l-methionine (SAM) synthase from E. coli K12 (MetK) with subsequent feeding of l-methionine and glycerol in the culture. The best condition produced ∼279 μM (88.2 mg/L) of tamarixetin. The biological activity of tamarixetin was tested and compared with quercetin, 7-O-methylated quercetin, and 3-O-methylated quercetin. Results showed that the growth of all tested cancer cell lines (AGS, B16F10, C6, and HeLa) were inhibited by tamarixetin more effectively than other methylated derivatives of quercetin or quercetin. Tamarixetin also exhibited the best antimelanogenic activity among all compounds tested.

中文翻译：

重组大肠杆菌中生物活性他莫西汀的生物合成

据报道，柽柳素是槲皮素的单甲基化衍生物，具有许多重要的生物活性。在本研究中，全细胞生物转化系统用于槲皮素的区域特异性甲基化，以使用来自Streptomyces sp. 的甲基转移酶生产4'- O-甲基化槲皮素（tamarixetin）。大肠杆菌Bl21 (DE3) 中的KCTC 0041BP 。通过添加含有来自大肠杆菌K12 (MetK) 的S-腺苷-l-甲硫氨酸 (SAM) 合酶的质粒，随后加入l- 培养物中的蛋氨酸和甘油。最佳条件产生了约 279 μM (88.2 mg/L) 的他马利西汀。对柽柳素的生物活性进行了测试，并与槲皮素、7- O-甲基化槲皮素和3- O-甲基化槲皮素进行了比较。结果表明，与槲皮素或槲皮素的其他甲基化衍生物相比，柽柳素对所有受试癌细胞系（AGS、B16F10、C6 和 HeLa）的生长抑制更有效。Tamarixetin 在所有测试的化合物中也表现出最好的抗黑色素生成活性。

更新日期：2020-05-19

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