Garcinia cambogia is one of the most commonly used anti‐obesity dietary supplements, and hydroxycitric acid (HCA) is a major constituent in the commercial preparations of Garcinia. High doses of HCA are often consumed without much awareness of its pharmacokinetic and toxicokinetic parameters, and therefore, a complete understanding of its effects is lacking. The first step in understanding these parameters is the availability of a reliable bioanalytical method. Here, we present the first report on a UPLC–MS/MS method for analysis of HCA in rat plasma after a simplified and cost‐effective protein precipitation. Chromatographic separation of the analytes in the supernatant was achieved using hydrophilic interaction liquid chromatography, where mass parameters were optimized and a rapid 5‐min quantitative assay was developed. The method was highly sensitive, accurate, precise and linear in the concentration range of 10.5–5000 ng/mL (validated according to the United States Food and Drug Administration guidelines). Further, the method was successfully used to describe the pharmacokinetic profile of HCA in rat plasma after the administration of pure HCA and commercial Garcinia preparations.

中文翻译：

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快速，经济高效的LC-MS / MS测定血浆中羟柠檬酸的方法：在商业藤黄制剂中药代动力学测定中的应用。

藤黄果是最常用的抗肥胖膳食补充剂之一，羟基柠檬酸（HCA）是藤黄商业制剂中的主要成分。通常在不了解其药代动力学和毒代动力学参数的情况下食用高剂量的HCA，因此缺乏对HCA效果的全面了解。了解这些参数的第一步是获得可靠的生物分析方法。在这里，我们提供了关于UPLC-MS / MS方法的第一份报告，该方法在简化且具有成本效益的蛋白质沉淀之后，用于分析大鼠血浆中的HCA。上清液中分析物的色谱分离是通过亲水作用液相色谱实现的，其中质量参数得到了优化，并开发了一种快速的5分钟定量分析方法。该方法在10.5–5000 ng / mL的浓度范围内是高度灵敏，准确，精确和线性的（根据美国食品和药物管理局的指导原则进行了验证）。进一步，藤黄制剂。