Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936.,Stem Cell Research

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Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936.
Stem Cell Research ( IF 0.8 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.scr.2020.101851
Jamie Toombs ₁ , Lindsay Panther ₂ , Loren Ornelas ₂ , Chunyan Liu ₂ , Emilda Gomez ₂ , Raquel Martín-Ibáñez ₃ , Simon R Cox ₄ , Stuart J Ritchie ₄ , Sarah E Harris ₄ , Adele Taylor ₄ , Paul Redmond ₄ , Tom C Russ ₅ , Lee Murphy ₆ , James D Cooper ₇ , Karen Burr ₇ , Bhuvaneish T Selvaraj ₇ , Cathy Browne ₈ , Clive N Svendsen ₉ , Sally A Cowley ₁₀ , Ian J Deary ₄ , Siddharthan Chandran ₇ , Tara L Spires-Jones ₁ , Dhruv Sareen ₁₁

Affiliation

Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK.
iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK; Alzheimer Scotland Dementia Research Centre, University of Edinburgh, Edinburgh, UK.
Edinburgh Clinical Research Facility, University of Edinburgh, Edinburgh, UK.
Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.
James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK; Oxford Parkinson's Disease Centre, Oxford, UK.
iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA; Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.

Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

中文翻译：

从 1936 年洛锡安出生队列的 24 名成员中产生 24 种诱导多能干细胞系。

认知能力下降是衰老过程中最令人担心的问题之一。我们从 1936 年洛锡安出生队列的 24 名参与者身上培育出了诱导多能干细胞 (iPSC)，他们的认知能力在儿童期和老年期进行了测试。使用表达六种 iPSC 重编程因子（OCT3/4 (POU5F1)、SOX2、KLF4、L-Myc、shp53、Lin28、SV40LT）的非整合 oriP/EBNA1 骨干质粒对外周血单核细胞 (PBMC) 进行重编程。所有品系都显示 STR 匹配的核型，并且通过多种方法验证了多能性。这些 iPSC 系是研究认知衰老个体差异和年龄相关神经退行性疾病恢复能力的分子机制的宝贵资源。

更新日期：2020-05-20

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