Abstract Bioactive natural metabolites, especially from the marine endophytic fungi, are largely unexplored. Endophytic fungi are being increasingly recognized as a group of organisms that produce novel metabolites of industrial importance. This study investigated the anticancer and antibacterial potential of the marine algal endophyte, Penicillium chrysogenum. The different organic solvent extracts of the endophytic fungi grown on different growth medium were analyzed for anticancer and antibacterial activities. The highest inhibitory activity was observed for the ethyl acetate (EA) extract of the culture filtrate grown in potato dextrose broth (PDB) for 21 days, against the tested human breast cancer cell (MCF-7) line. Similarly, the PDB-EA extract showed an appreciable activity against the human pathogens. The biochemical analysis of the Cha EA metabolites revealed terpenoids, steroids, phenolics and flavones. Gas Chromatography (GC MS) data revealed several bioactive compounds such as anthraquinone and cinnamic acid. The Cha EA extract induced membrane damage and thus, apoptosis in MCF-7cells. The secondary metabolites produced by these marine endophytic fungi have contributed to considerable anticancer and antimicrobial activities and hence, this study is an evidence of potential sources of antimicrobial and anticancer compounds from Penicillium chrysogenum.

中文翻译：

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来自绿藻的海洋内生真菌的分子分析：抗菌和抗癌活性的评估

摘要 具有生物活性的天然代谢物，特别是来自海洋内生真菌的，在很大程度上尚未开发。内生真菌越来越多地被认为是一组能够产生具有工业重要性的新型代谢物的生物体。本研究调查了海洋藻类内生菌 Penicillium chrysogenum 的抗癌和抗菌潜力。分析了生长在不同培养基上的内生真菌的不同有机溶剂提取物的抗癌和抗菌活性。对于在马铃薯葡萄糖肉汤 (PDB) 中生长 21 天的培养物滤液的乙酸乙酯 (EA) 提取物，观察到对测试的人乳腺癌细胞 (MCF-7) 系具有最高的抑制活性。类似地，PDB-EA 提取物对人类病原体显示出明显的活性。Cha EA 代谢物的生化分析揭示了萜类、类固醇、酚类和黄酮。气相色谱 (GC MS) 数据揭示了几种生物活性化合物，如蒽醌和肉桂酸。Cha EA 提取物诱导膜损伤，从而诱导 MCF-7 细胞凋亡。这些海洋内生真菌产生的次生代谢产物具有相当大的抗癌和抗菌活性，因此，本研究证明了产黄青霉抗菌和抗癌化合物的潜在来源。